Farenimio
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Isolation of Cholesterol?
Does anyone knows how to isolate cholesterol from... something... anything... that doesn't involve extracting with a chromatography column... I don't
have access to that fancy stuff.
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JJay
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I have seen canisters of cholesterol sold as a hair styling product. You might be able to get it from beauty salons. It is easy to purchase from
chemical suppliers but I don't remember which ones sell it offhand.
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Dr.Bob
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We did the extraction of cholesterol from gallstones when I was in college. That worked pretty well, and is in one of the introductory org chem lab
books, but I can't remember which off hand. But a quick search in google found many versions, plus also ones using egg yolk, which should be easier
to get hold of at the store, unless you should at a pretty odd market.
https://www.google.com/?gfe_rd=ssl&ei=zoe_VqnqJcPA-AWni6...
https://www.google.com/?gfe_rd=ssl&ei=zoe_VqnqJcPA-AWni6...
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Tsjerk
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Brain tissue is apparently a good source, maybe a butcher around?
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CuReUS
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Quote: Originally posted by Dr.Bob  | | We did the extraction of cholesterol from gallstones when I was in college. That worked pretty well, and is in one of the introductory org chem lab
books, but I can't remember which off hand. |
I think you are referring to this book,which is there in the SM library
http://library.sciencemadness.org/library/books/Experiments_...
[Edited on 14-2-2016 by CuReUS]
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Tsjerk
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Prep Biochem. 1977;7(3-4):181-6.
A simple and rapid method for the isolation of cholesterol from spinal cord of cattle.
Ali SA, Jamal N.
Maybe something like this? I don't have excess, but maybe you can ask in the reference section.
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Nicodem
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Thread Moved
14-2-2016 at 07:19 |
Crowfjord
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I also isolated cholesterol from human gallstones as a college organic chemistry exercise. Pure crystalline cholesterol is actually quite beautiful.
Soft, sparkling, pearly stuff.
The procedure consisted of boiling crushed gallstone in 2-butanone (MEK), filtering to remove bilirubin and bile pigments, and evaporating about two
thirds of the solvent. The concentrated solution is diluted with a little methanol, heated to boiling, then water is added drop-wise until a very
faint cloudiness persists. The vessel is insulated and the solution allowed to cool slowly to ambient temperature, then further cooled in ice. The
crystals are vacuum filtered, washed with a little ice-cold methanol, then allowed to dry.
The above extracted cholesterol still contains small amounts of cholestanol, cholestadienol, and cholestenol, which can be separated by bromination.
The dibromo derivative of cholesterol is sparingly soluble, whereas the above contaminants either don't react or are dehydrogenated by the bromine, so
stay soluble. The dibromo derivative is filtered and debrominated with zinc dust in ether/acetic acid.
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Metacelsus
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How does one obtain gallstones?
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j_sum1
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I think a poor diet helps. 
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Crowfjord
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I think the ones we had at school were donated from a local hospital. Maybe you can get some if you say they are for educational use?
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