Separating/excluding lactose from lactulose
Hello fellow sciencemadness users! I've been lurking here for some time, and I've finally decided to create a post that has piqued some of my
chemical curiosity.
I've been investigating a seemingly plausible route to lactulose from lactose using an applied DC in the range of one or two-hundred mA ( http://www.sciencedirect.com/science/article/pii/S1466856412... ). Now, I'm not sure how this amperage would be controlled with readily available
things, like a car battery charger for instance, but I guess this was not the initial intent of this post. Instead, what I'm wondering is how
separation of lactose, lactulose, and galactose would go. (I have not personally investigated this "electro-isomerization, and this is somewhat
beyond my academic skill level). I am definitely interested in learning the theory if anyone can help me out.
Another article I've found describes using an "ion-exchange resin" chromatography column (I still don't know what this Ion-exchange resin stuff is)
for separation. ( http://link.springer.com/article/10.1007/BF02688086?no-acces... "Study of the separation of lactose, lactulose and galactose by liquid
chromatography using cationic ion-exchange resin columns.")
The first article mentioned just simply states "excluding" of lactose and other sugars, but I'm wondering how this is generally done, if there are
other ways (By excluding lactose, the end product purity was 96.28 ± 0.18%, which is similar to the pharmakopoeia requirements for lactulose powder.)
Any additional information on controlling the amperage and choosing a power supply would also be greatly appreciated (have some for computers and
charging/jumping batteries. I would also like to add that while I don't plan on consuming this at all, I would like to keep this (theoretically)
relatively pure. Thanks, in advance, for any help on this subject.
[Edited on 26-10-2015 by SiC]
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