memeticist
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Buffer for redox sensitive reduced glutathione
Hello,
I was wondering what would be a safe basic buffering agent for a glutathione solution. I saw in one write up that octylammonium orthophosphate would
be suitable. But, I was wondering if plane Jane ammonium phosphate would be acceptable?
Thanks in advance
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Crowfjord
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My guess is yes. I did some cyclic voltammetry experiments with glutathione in sodium phosphate buffer a few years ago. I doubt the ammonium would
change things very much. Google "glutathione buffer cyclic voltammetry" or "glutathione buffer redox" or something like that. Should give you some
ideas.
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phlogiston
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Should be ok. I have very often used TRIS for buffering GSH solutions to around pH 8.0.
If you are using it for biochemistry/enzymatic experiments, do consider that some buffering agents can affect the activity of your enzyme. If an
unusual buffer is used in a paper, this could be the reason and you should be aware of that.
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"If a rocket goes up, who cares where it comes down, that's not my concern said Wernher von Braun" - Tom Lehrer |
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