Boba155
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Question about Column Chromatography and Elution Gradient
Hey all,
Just wondering a quick question. A formula I am using calls for me to perform an elution gradient in a column (25%, 50%, 75%, and 100% ethyl acetate
in benzene) in order to extract a compound from tissue.
However the amount of benzene the process calls for is quite staggering (over 20 liters). I can't exactly pay for all this benzene.
Anyway, my question is, do you think I could use a cheaper solvent, such as Toluene or Hexane? In the same amounts? (i.e. if it calls for 5L of 25% EA
in Benzene, I just use 5L of 25% EA in Hexane)
Thanks all!
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antibody
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if you want a substitute for benzene, then you want a solvent that is more polar than hexane. xylene or diethyl ether would be better substitutes.
Next best would be DCM or Toluene.
Benzene is a lot more polar than hexane.
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