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Author: Subject: Extraction microrna From Humans Plasma
vegafelisa
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[*] posted on 20-10-2019 at 19:33
Extraction microrna From Humans Plasma


Hi. My question is: what is the best way for extraction microrna from humans plasma? Whether trizol is suitable, or i will should use commercial kits? Thanks
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Metacelsus
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[*] posted on 21-10-2019 at 04:02


Trizol should work, but under some conditions low GC miRNAs could be lost. Make sure to use appropriate controls. See the attached references.

Attachment: PIIS1097276512004819.pdf (350kB)
This file has been downloaded 564 times

Attachment: s12864-015-1299-5.pdf (666kB)
This file has been downloaded 546 times

Also this thread should probably be moved to the Biochemistry subforum.

[Edited on 2019-10-21 by Metacelsus]




As below, so above.

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21-10-2019 at 05:54
vegafelisa
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[*] posted on 29-10-2019 at 22:03


Thank you so much, really helpful these two attachments. I will keep your suggestion in mind next time I have any questions.
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