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White Yeti
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Definitely, go right ahead! Be sure to post a procedure on how you did it.
I am currently growing a liquid culture of Trametes versicolor. After that, I'm going to have to use PF Tek, a technique I've never tried
before to get the fungus to colonise a slab of growth medium. I was planning on modifying the technique a bit by directly injecting mycellium into the
jar instead of messing around with spore syringes and what not. In any case, this fungus does not propagate nearly as quickly as the penicillium
(which is to be expected). I won't be 100% sure I have the right fungus until it starts to fruit. All mycelia look alike, white and fuzzy.
"Ja, Kalzium, das ist alles!" -Otto Loewi
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Eddygp
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My LC Fungi
Surprising results... From a small patch of fungi, I have now dozens of little mycelium dots scattered all over the LC, apart from the two larger
blobs.
Add some green food colouring to tap water. Add 5 drops of vinegar and 2 drops of diluted starch. Add sugar in an aqueous solution (as much as you
want). Inoculate some spores.
I'm going to cultivate some macroscopic fungi, probably Fomes, Trametes or Grifola species, but I will do first some research on spore
syringes.
[Edited on 14-4-2012 by Eddygp]
[Edited on 14-4-2012 by Eddygp]
there may be bugs in gfind
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ssdd
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Hi Eddygp,
This looks very nice and is likely a great media for growing moulds, many of which are oligotrophic and will not require many nutrients (I've seen
some grow on nothing more than molecular grade agarose and ultrapure water). But for growing larger species like you are hoping to try it likely won't
work since these are going to require large amounts of nutrients other than fermentable carbon. A good complex media that may work and is very easy to
make is PDA (Potato Dextrose Agar) http://en.wikipedia.org/wiki/Potato_dextrose_agar this can be used as a broth as well since you don't have agar, and sucrose can replace the
dextrose. The potatoes used in making this media will provide all the other nutrients needed.
Good luck with your fungi cultivation and keep us posted!
-ssdd
All that glitters may not be gold, but at least it contains free electrons.
-- John Desmond Baernal
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Eddygp
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Thank you, ssdd!
I have been thinking of growing saprotrophic fungi, and will probably use that agar. However, I have also thought of growing them on wood. I have read
that many saprotrophic fungi prefer mildly acidic conditions (which are quite easy to make, adding a bit of vinegar). Thank you for the idea!
Eddy
PS. Would flour be useful to subtitute the agar?
[Edited on 16-4-2012 by Eddygp]
there may be bugs in gfind
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White Yeti
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@Eddygp, sorry for the late reply, but that culture looks fantastic! I like your idea to use food colouring to show the fungus better. I didn't try
adding vinegar either, but this is definitely something I'll be trying soon. I think I'll try to grow Agaricus bisporus (Portobello mushroom)
with the PF-tek technique instead of Trametes versicolor because it seems like my culture failed Either I contaminated the plate, or another fungus is feeding off of the fungus I introduced into the growth medium. I
don't know for sure, so that's why I'm starting over.
"Ja, Kalzium, das ist alles!" -Otto Loewi
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ssdd
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Dunno if flour would work, it might do OK, but I could see it being a real pain to sterilize to any level (unless you have a pressure cooker or
something).
Something else that might work (just throwing some untested ideas out here) is using a solid substrate like pearlite and soaking liquid media onto
them. But then pearlite would also be difficult to sterilize...
Let us know what you find that works!
I do know of a few exoctic, but probably hard to get, agar substitutes: Gellan Gum (works nicely, but needs +2 ions to solidify, once solid you can't
really melt it again), Polyvinyl Alcohol (makes a nice think syrup), Gelatin of course but its easily broken down, etc...
-ssdd
All that glitters may not be gold, but at least it contains free electrons.
-- John Desmond Baernal
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Eddygp
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My fungi are growing very quickly!
there may be bugs in gfind
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Eddygp
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Anyone still interested in this topic?
there may be bugs in gfind
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White Yeti
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Yes. Progress is slow and rather uneventful, yet ongoing. I'm working on a growth medium from a corn starch, egg whites, sucrose, food colouring, and
various micronutrients (iron, zinc, copper). Dosing micronutrients is very important, too much iron and nothing grows. I'll also add a little vinegar
to lower the pH.
At the moment I'm swamped, I'm preparing for four AP exams, no free time whatsoever.
"Ja, Kalzium, das ist alles!" -Otto Loewi
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Eddygp
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Good luck! My fungi withered away by a factor I still do not understand... I'll see if this autumn I get some small saprotrophic fungi to grow on a
sucrose-flour-sawdust mixture I'm planning.
there may be bugs in gfind
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Eddygp
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I'm leaving this for a while. In October or so, I will try to get some small err... saprotrophic fungi to cultivate from spores.
there may be bugs in gfind
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Random
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did you manage to do anything eddygp?
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Eddygp
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Actually, I did. But not exactly what I was intending to do... -.-
In a nutshell...
I tested many fungicides and antibiotics on some fungi cultures I had, as well as bacteria. I also compared them wit ha slice of freshly cut garlic.
Umm, the fungicides worked just as I expected, with low concentration being very harmful. The antibiotics I used only left some strange boneish white
bacteria colonies (very very small). The garlic slice had made a circle with radius ~ 0.9 cm with no bacteria or fungi. However, some very interesting
orange colonies of bacteria had grown... on top of the garlic!!! These bacteria have developed some sort of resistance to the substances garlic has,
and they seem to grow only there. I guess garlic should some day be decomposed or the world would be full of garlic slices... 
Yeah, just about that, without speaking of the crickets (Toby, Sophie, August and Tora (long story)) or the fungi identification (the biology dep.
didn't have any species for its collection, they only had some shrews, frogs, etc.).
there may be bugs in gfind
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magnus454
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Anyone try polyacrylamide crystals? I've doped them with a little miracle grow for plant growth medium experiments, and had fungus outbreaks on them,
so I had to add a small amount of con-san to the mix to prevent it. biodegrades into ammonia and nitrogen gas.
History is repeating itself.
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Eddygp
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Hmm I haven't tried those.
there may be bugs in gfind
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Eddygp
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I have made a spore print of an Inocybe species
there may be bugs in gfind
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