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mayko
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Location: Carrboro, NC
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Another Prakash Lab invention: the HandyFuge, based on the principle of a squeeze-charge flashlight
Handyfuge-LAMP: low-cost and electricity-free centrifugation for isothermal SARS-CoV-2 detection in saliva.
Ethan Li, Adam Larson, Anestha Kothari, Manu Prakash
medRxiv 2020.06.30.20143255; doi: https://doi.org/10.1101/2020.06.30.20143255
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Point of care diagnostics for COVID-19 detection are vital to assess infection quickly and at the source so appropriate measures can be taken. The
loop-mediated isothermal amplification (LAMP) assay has proven to be a reliable and simple protocol that can detect small amounts of viral RNA in
patient samples (<10 genomes per μL) (Nagamine, Hase, and Notomi 2002) Recently, Rabe and Cepko at Harvard published a sensitive and simple
protocol for COVID-19 RNA detection in saliva using an optimized LAMP assay (Rabe and Cepko, 2020). This LAMP protocol has the benefits of being
simple, requiring no specialized equipment; rapid, requiring less than an hour from sample collection to readout; and cheap, costing around $1 per
reaction using commercial reagents. The pH based colorimetric readout also leaves little ambiguity and is intuitive. However, a shortfall in many
nucleic acid-based methods for detection in saliva samples has been the variability in output due to the presence of inhibitory substances in saliva.
Centrifugation to separate the reaction inhibitors from inactivated sample was shown to be an effective way to ensure reliable LAMP
amplification. However, a centrifuge capable of safely achieving the necessary speeds of 2000 RPM for several minutes often costs hundreds of dollars
and requires a power supply. We present here an open hardware solution- Handyfuge - that can be assembled with readily available components for the
cost of <5 dollars a unit and could be used together with the LAMP assay for point of care detection of COVID-19 RNA from saliva. The
device is then validated using the LAMP protocol from Rabe and Cepko. With the use of insulated coolers for reagent supply chain and delivery, the
assay presented can be completed without the need for electricity or any laboratory scale infrastructure.
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al-khemie is not a terrorist organization
"Chemicals, chemicals... I need chemicals!" - George Hayduke
"Wubbalubba dub-dub!" - Rick Sanchez
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Ubya
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Location: Rome-Italy
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Mood: I'm a maddo scientisto!!!
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Quote: Originally posted by mayko  | Another Prakash Lab invention: the HandyFuge, based on the principle of a squeeze-charge flashlight
Handyfuge-LAMP: low-cost and electricity-free centrifugation for isothermal SARS-CoV-2 detection in saliva.
Ethan Li, Adam Larson, Anestha Kothari, Manu Prakash
medRxiv 2020.06.30.20143255; doi: https://doi.org/10.1101/2020.06.30.20143255
| Quote: |
Point of care diagnostics for COVID-19 detection are vital to assess infection quickly and at the source so appropriate measures can be taken. The
loop-mediated isothermal amplification (LAMP) assay has proven to be a reliable and simple protocol that can detect small amounts of viral RNA in
patient samples (<10 genomes per μL) (Nagamine, Hase, and Notomi 2002) Recently, Rabe and Cepko at Harvard published a sensitive and simple
protocol for COVID-19 RNA detection in saliva using an optimized LAMP assay (Rabe and Cepko, 2020). This LAMP protocol has the benefits of being
simple, requiring no specialized equipment; rapid, requiring less than an hour from sample collection to readout; and cheap, costing around $1 per
reaction using commercial reagents. The pH based colorimetric readout also leaves little ambiguity and is intuitive. However, a shortfall in many
nucleic acid-based methods for detection in saliva samples has been the variability in output due to the presence of inhibitory substances in saliva.
Centrifugation to separate the reaction inhibitors from inactivated sample was shown to be an effective way to ensure reliable LAMP
amplification. However, a centrifuge capable of safely achieving the necessary speeds of 2000 RPM for several minutes often costs hundreds of dollars
and requires a power supply. We present here an open hardware solution- Handyfuge - that can be assembled with readily available components for the
cost of <5 dollars a unit and could be used together with the LAMP assay for point of care detection of COVID-19 RNA from saliva. The
device is then validated using the LAMP protocol from Rabe and Cepko. With the use of insulated coolers for reagent supply chain and delivery, the
assay presented can be completed without the need for electricity or any laboratory scale infrastructure.
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this is ok for 0.5-1.5ml tubes, i would not try this with my 15ml glass centrifuge tube
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feel free to correct my grammar, or any mistakes i make
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Mateo_swe
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Hmm, i would like a centrifuge with 1000ml bottles.
I guess it could be done with some DIY experimentation.
I dont need any 1000 of rpms, i would guess bike wheel speed would be enough.
And at these speeds it would be easier to shield and a breakdown would only damage the centrifuge and samples.
It would be nice for hard to filtrate stuff.
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draculic acid69
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| Quote: Originally posted by ScienceSquirrel |
That looks more like a weapon than a piece of scientific equipment. Imagine turning up at your local hospital with a piece of PVC tube embedded in
your chest.  |
That thing looks dangerous.
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Tsjerk
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I laughed at the PRECAUTIONS section 
| Quote: | | ....if possible use a protective shield, ex: a riding helmet...... |
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draculic acid69
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I laughed at the PRECAUTIONS section
| Quote: | | ....if possible use a protective shield, ex: a riding helmet...... |
[/rquote]
Yeah that'll protect you when six weighted swinging pvc tubes spinning at a few thousand Rpm's smack into your arm,body,neck,head & face
[Edited on 10-7-2020 by draculic acid69]
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Eddie Current
Hazard to Self
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| Quote: Originally posted by Mateo_swe | Hmm, i would like a centrifuge with 1000ml bottles.
I guess it could be done with some DIY experimentation.
I dont need any 1000 of rpms, i would guess bike wheel speed would be enough.
And at these speeds it would be easier to shield and a breakdown would only damage the centrifuge and samples.
It would be nice for hard to filtrate stuff. |
How close do you live to a playground?
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SWIM
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Cream separators, anybody?
A nice hand -cranked DeLaval #18 maybe?
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draculic acid69
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In Australia we used to have hills hoist.it has a hand crank halfway up the middle pole.
One could attach many coke bottles or a few 20litre drums with some reinforcement if desperate/stupid enough to think it's a good idea
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Mateo_swe
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Location: Within EU
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Something like this looks interesting.
It would need a sturdy shield.
And the playarea for children where i live dont have a merry-go-round (thats what they are called, right?)
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SWIM
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Registered: 3-9-2017
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| Quote: Originally posted by draculic acid69 | In Australia we used to have hills hoist.it has a hand crank halfway up the middle pole.
One could attach many coke bottles or a few 20litre drums with some reinforcement if desperate/stupid enough to think it's a good idea
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Don't sell the hills hoist short.
They were all over US suburbs in the 60s and 70s too.
Some housing developments mandated their use because they didn't want clotheslines hanging all over the place.
They probably sold hundreds of thousands here. Maybe a million.
Gee, I wonder if spinning it like a centrifuge would save much time on drying your clothes?
I suppose your socks might get a little longer with time though.
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draculic acid69
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Posts: 1371
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Don't sell the hills hoist short.
They were all over US suburbs in the 60s and 70s too.
Some housing developments mandated their use because they didn't want clotheslines hanging all over the place.
They probably sold hundreds of thousands here. Maybe a million.
Gee, I wonder if spinning it like a centrifuge would save much time on drying your clothes?
I suppose your socks might get a little longer with time though.[/rquote]
I'm wondering how much weight you can hang off one before it goes bad?
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