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Brain&Force
Hazard to Lanthanides
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Location: UW-Madison
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KRISTOF WITH A LIGHTSABER! OH YEAH! Now that's what I'm talking about!
Here are some bacteria I transformed. I don't know why the image is rotated.
<img src="http://i.imgur.com/gImIFam.jpg" title="I kept calling them my bacteria babies. That seemed to really creep everyone out." width=800>
My friends got better results:
<img src="http://i.imgur.com/u10WFLo.jpg" title="When the alt-text reaches a certain length, it seems to just enter a line break for some odd
reason, like here. Anyone know why?" width=800>
At the end of the day, simulating atoms doesn't beat working with the real things...
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confused
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Nice, Im guessing the green is GFP. 
Whats the blue and red protein?
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kristofvagyok
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I always wanted to work with those fluorescent things, they look adorable. Will you isolate the fluorescent protein from the cells?
On the pics: a little carbohydrate chemistry, 1,2:3,4:5,6-Tri-O-isopropylidene-D-mannitol what was made directly from mannitol and acetone with a
little acid catalyst.
If you click on the pictures, you'll reach my portfolio, best pics from the past 2 years of the lab. Extra note: today I will post the 800.-th pics on
my blog, so if anyone is bored here is a random post: http://labphoto.tumblr.com/random
I have a blog where I post my pictures from my work: http://labphoto.tumblr.com/
-Pictures from chemistry, check it out(:
"You can’t become a chemist and expect to live forever." |
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Brain&Force
Hazard to Lanthanides
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Location: UW-Madison
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Mood: Incommensurately modulated
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Different colors are different mutants of GFP. We were given unlabeled plasmid mixes, so we didn't know what colors we'd get when we transformed the
bacteria.
I have no idea where the bacteria are now, and I don't think we're going to isolate the GFP from them. I have the procedure somewhere if you're
interested (though you will need to have the correct plasmid and some E. coli available).
[Edited on 20.4.2014 by Brain&Force]
At the end of the day, simulating atoms doesn't beat working with the real things...
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Brain&Force
Hazard to Lanthanides
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Location: UW-Madison
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<img src="http://i.imgur.com/b69MvRl.png" title="Might try it with boric acid next time." width=800>
"Thunder flasks" demo with methanol. Some copper chloride was added to color the flame, but with no success this time. (This is odd because my
procedure had worked for the previous trial.)
<img src="http://i.imgur.com/TEXCsQy.jpg" title="I'm not sure which species this sample came from." width=800>
Stomata were viewed through a microscope by taking nail polish and drying it on the underside of a leaf to make a cast, then wet mounted on a slide.
At the end of the day, simulating atoms doesn't beat working with the real things...
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Oscilllator
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Making sulfur chlorides:
<a href="http://imgur.com/bUheqrS"><img src="http://i.imgur.com/bUheqrS.jpg?1" title="Hosted by imgur.com"/></a>
<a href="http://imgur.com/mBZluZN"><img src="http://i.imgur.com/mBZluZN.jpg?1" title="Hosted by imgur.com"/></a>
<a href="http://imgur.com/tcHHo4J"><img src="http://i.imgur.com/tcHHo4J.jpg?1" title="Hosted by imgur.com"/></a>
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HeYBrO
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That must of been hard to clean and dispose.
[Edited on 25-4-2014 by HeYBrO]
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Oscilllator
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Yeah my glassware still has what looks like sulfur powder on it. I'm thinking of blasting it in my over to try and burn the sulfur. Got any tips on
how else I might go about it?
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HgDinis25
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Mood: Who drank my mercury?
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Quote: Originally posted by Oscilllator  | | Yeah my glassware still has what looks like sulfur powder on it. I'm thinking of blasting it in my over to try and burn the sulfur. Got any tips on
how else I might go about it? |
Hot Toluene readly dissolves Sulfur (I've done it my self). Carbon Disulfide could also bee used...
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The Volatile Chemist
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GFP is definitely cool Anyone tried mutating bacterium who have the gene? Would
be interesting... I don't have anything for such a procedure, but would love to see someone try! Maybe give them some light carcinogen...? If such a
thing exists
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Ionic Chemist
Harmless
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Gold Pictures
They might not be the best but I still consider this small collection pretty. All the pictures are from my recent attempts at gold purification via
liquid-liquid extraction, with isopropyl alcohol as the secondary component in a water based mixture.
1). Separation of gold laden organic phase (left), from copper based aqueous impurities (right).
2). Addition of calcium chloride (layering inducer).
3). Formation of a biphasic layer accompanied by the production of calcium carbonate (from trace impurities of sodium carbonate used in processing).
4). Preparation for transfer of gold laden organic phase for further testing.
5). Microscopic view of reduced gold in a capillary tube.
6.) View of gold deposits in a separate section of the capillary tube.
"Discoveries are not made by idly sitting around and hoping something interesting might happen; they are made
by getting out there and doing something to push the results and odds in your favour." "Chemistry always works... just not always in the way you
want."
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Etaoin Shrdlu
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I really like that second-to-last picture, Ionic Chemist. Taken with a better camera it would probably be magnificent.
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Zyklon-A
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Can anyone guess what this is?
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DraconicAcid
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Dilute manganate ion with dry ice.
Please remember: "Filtrate" is not a verb.
Write up your lab reports the way your instructor wants them, not the way your ex-instructor wants them.
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Töilet Plünger
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Bromothymol blue indicator with dry ice.
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The Volatile Chemist
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Take It one step farther... Green Dye With dry ice...
Just Kidding. Though that definitely looks like something sublimating.
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Töilet Plünger
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Nickel ion with dry ice.
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Manifest
Script Kiddie Asshole
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I would imagine dry ice would float though.
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Zyklon-A
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Ok, nickel ion is my guess too. I didn't take this picture, and I don't know what it is. I saw it here, and wanted to see if you guys could makes some guesses.
I have been wanting to make a thread, on guessing a chemical based on a picture. Or is there already such a thread.
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elementcollector1
International Hazard
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Pretty sure there is, somewhere in Whimsy. Something like "Where's Waldo for chemicals"...
Elements Collected:52/87
Latest Acquired: Cl
Next in Line: Nd
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Zyklon-A
International Hazard
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Mood: Fluorine radical
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Yes, I saw that, but isn't that where you try to guess where it is on the shelves?
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MrHomeScientist
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That's definitely dry ice, and almost certainly just green food coloring. Occam's razor, and all. I've done this a thousand times. Dry ice doesn't
float.
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kristofvagyok
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Not strictly chemistry, but:
Two pieces of fluorite under UV light.
Fluorite (CaF2) is naturally occurring mineral, it is the far most common and well-known fluorine containing mineral found on earth. When pure, it is
completely colorless and transmits light from 200 nm what means it lets through UVA, UVB and some of the UVC photons.
But when it is contaminated with other elements, usually with yttrium, cerium, iron, sodium, barium, aluminium, ect. it could change the color of the
mineal and could also cause a fluorescence under UV light, just as in this case. Originally the fluorite on the right had a green color, but it emits
strong blue light under UVB.
I have a blog where I post my pictures from my work: http://labphoto.tumblr.com/
-Pictures from chemistry, check it out(:
"You can’t become a chemist and expect to live forever." |
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smaerd
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Just out of curiousity Kristof how much do UV lamps like that light saber tend to cost? Euros or anything is fine. I was trying to think of a way to
make one without shelling out tons and tons of money but I'm not sure it's practical.
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Brain&Force
Hazard to Lanthanides
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I have some fluorite, but it doesn't fluoresce at all. But I have some corundum that fluoresces red - if I find it I'll post a photo of it under UV.
smaerd, if it works for you, you can buy an ultraviolet (black light) compact fluorescent lamp from a party store (in the US Party City is one place.
Most work fine, though there are a few duds that don't actually emit UV light. You can check by holding a US $20 bill behind the light
Here's potassium terbium sulfate fluorescing in a beaker, after separation from an iron-containing solution. Iron REALLY kills terbium fluorescence
(which is why some of the crystals aren't as bright).
<img src="http://i.imgur.com/69Gz2yl.jpg" title="I just won $500 in the lottery and a $2500 scholarship. Amazing huh?" width=800>
[Edited on 15.5.2014 by Brain&Force]
At the end of the day, simulating atoms doesn't beat working with the real things...
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