RfoG3282
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protein pKa determination
Hello!
I am trying to determine the pKa of hemoglobin using UV/Vis spectrophotometry and a pH meter. I've found that I can do this by recording the changes
in the spectra. I've found that I can get the pKa with a modified Henderson-Hasselbach equation but I can't quite get the math to work out. Can anyone
with knowledge of the subject tell me how I should go about this?
Thanks!!
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Hockeydemon
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Quote: Originally posted by RfoG3282  | Hello!
I am trying to determine the pKa of hemoglobin using UV/Vis spectrophotometry and a pH meter. I've found that I can do this by recording the changes
in the spectra. I've found that I can get the pKa with a modified Henderson-Hasselbach equation but I can't quite get the math to work out. Can anyone
with knowledge of the subject tell me how I should go about this?
Thanks!! |
Can you post the figures your using? When you say a modified version of the H-H equation are you referring to an actual variation or just the
rearrangement to solve for pKa?
Hemoglobin K = 1.4 x 10-8; pKa = 7.85
Without knowing your figures though I'm (personally) unable to help you.
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