soma
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after packing chromatography column
Would there be a problem if after packing the column (for gravity or flash) it is left for many hours before using it? (Keeping a layer of solvent
above the silica).
(I had posted this in another thread with a different subject but thought it would to better to have its own subject.)
[Edited on 13-1-2017 by soma]
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Tsjerk
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Should be no problem as long as you don't have a gradient in the column.
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Metacelsus
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It's fine.
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phlogiston
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As Metacelcus and Tsjerk already said: thats perfectly fine. You can store it like that indefinitely. However, do avoid significant temperature
fluctuations. That could cause dissolved gasses to 'precipitate' (I suspect that's not the proper word for a gas, suggestions?) forming bubbles that
will ruin your nice packing.
[Edited on 14-1-2017 by phlogiston]
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CharlieA
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effervesce
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PHILOU Zrealone
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Quote: Originally posted by phlogiston  | That could cause dissolved gasses to 'precipitate' (I suspect that's not the proper word for a gas, suggestions?) forming bubbles that will ruin your
nice packing.
[Edited on 14-1-2017 by phlogiston] |
"Degas/degassing", "bubbles/bubbling", "boils/boiling" and "bubble coalescence" are what you are after I think 
PH Z (PHILOU Zrealone)
"Physic is all what never works; Chemistry is all what stinks and explodes!"-"Life that deadly disease, sexually transmitted."(W.Allen)
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CharlieA
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The release of dissolved gases (the subject under discussion) is not the same as "boils/boiling." "Degas/degasing" and effervescence are relevant
terms to describe the release of gases from a solution.
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phlogiston
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Thanks guys, looks like 'to effervesce' is indeed the verb I was looking for. Added another useful word to my vocabulary.
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JJay
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How much material can be reasonably separated in a 50 mL burette? I realize that there's probably no one answer for this, but is there a rule of thumb
I could use?
I'd hate to waste any material that I considered valuable enough to chromatograph....
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