soma
Hazard to Others
Posts: 297
Registered: 31-7-2010
Member Is Offline
Mood: No Mood
|
|
best method to analyze amino acids
It looks like I've succeeded in synthesizing n-acetylcysteine amide using microwave synthesis. (Verified by tlc.)
I see that others have used hplc to verify the product (like Alcrich).
Would that be the best method? I've got access to a mass spec and also nmr.
|
|
|
phlogiston
International Hazard
Posts: 1379
Registered: 26-4-2008
Location: Neon Thorium Erbium Lanthanum Neodymium Sulphur
Member Is Offline
Mood: pyrophoric
|
|
If all you want is to confirm that your product is what you think it is, NMR and MS are good techniques.
HPLC would more helpful if you want to investigate the purity of your product.
BTW, your compound can probably form disulfide bridges, so in your analysis make sure to pay attention the the possible presence of the dimerized
product, and/or, if possible, reduce the disulfides before analysis.
-----
"If a rocket goes up, who cares where it comes down, that's not my concern said Wernher von Braun" - Tom Lehrer |
|
|
![[*]](images/xpblue/default_icon.gif){kind=icon}
