Waffles SS
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Solvent for Auxins
I want to make mixed solution of 3 different Auxin and keep this solution for long time.but i dont know what solvent is suitable and safe for all of
them.
My Auxins are Indole butyric acid ,Naphthyl acetic acid,Gibberellic acid
For better solving I tried Potassium salt of these(i added these auxins to potassium hydroxide solution )but i read some where that Gibberellic acid
decompose in Aqueous solution.
Then i tried to solve these auxins in EtOH(96%) but after awhile the color of solution turned dark and terrible odor produced(i think that should be
ester of IBA or NAA)
what about acetone?
Someone has suggestion?
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smaerd
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Mood: hmm...
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The solvent may not be the concern. It's degredation that is the problem. Do you know how these solutions degrade?
Is it light, oxygen, chlorine in the tap water, etc? Do they polymerize, oxidize. etc?
Here's my advice. Make the the solvent is relatively free of impurities. Store in an amber bottle using a degassed solvent. Purge the bottle with CO2,
N2, or Ar. Store in a cold place.
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Waffles SS
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Thanks smaerd,
Sure i use degassed Distilled water but Gibberellic acid decompose in water(even in degassed water or in amber bottle)
somebody know what is suitable solvent for these auxin?
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Paddywhacker
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10% ethanol in dimethyl sufoxide (DMSO) and store it in a fridge.
The ethanol is there to stop the DMSO from freezing solid, and DMSO is, like ethanol, a disinfectant, so moulds or bacteria cannot grow in your
solution and degrade it. Also, DMSO is kinder to your plants than ethanol.
If you store an aqueous solution then you risk bacteria and moulds.
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Protium1
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Paddywhacker's solution is probably a safe place to start from. I would also advise to keep solution protected from light, heat and also oxygen. Maybe
wrap the container in aluminum foil and keep stoppered tight in the fridge.
Gibberelic acid has a hydroxyl group that can oxidize to a ketone, but also has a double-bond Beta to the ketone, so may be oxidized further. Perhaps
a mild antioxidant such as Vitamin C (ascorbic acid) might be used in conjunction, or a protective layer of nitrogen in the storage container might
help.
IF DMSO cannot be had, I'm sure EtOH would work just fine if kept cold, dark, and protected from too much atmospheric oxygen. Or anhydrous
iso-propanol can be found at any well-stocked pharmacy.
[Edited on 8/17/2013 by Protium1]
[Edited on 8/17/2013 by Protium1]
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