Sciencemadness Discussion Board

solubility

soma - 28-10-2016 at 22:14

I recently received a sample of n-acetylcysteine amide to use as a standard for analysis. It came with specs that said it's solubility is "water to 100 mM".

The mw for it is 162.21. I am thinking they mean that up to 16.22 gms of the substance is soluble in lL of water? They also, in general, recommend using a 100mM solution of NaOH to help to dissolve amino acids.


Dwarvensilver - 4-11-2016 at 12:48

Well Soma,
I am a noob and definitely not tops in chemistry knowledge ( there is just so much :)).
I came across this web page on molar equivalents that may answer your query.

https://www.tocris.com/technicalSupport.php?Item=molarEquiva...


Cheers,

Dwarvensilver

zed - 4-11-2016 at 12:54

Wouldn't use NaOH without doing some research.

The material you are using, is the Amide of an amino-acid. That amide could be hydrolysed under basic conditions.

Tsjerk - 5-11-2016 at 12:18

Your calculation is correct, if the supplier recommends 100 mM NaOH I don't see a reason not to use it. But maybe make a fresh solution every time you need it for reasons mentioned by zed.