I was in the lab yesterday making a buffer with the flu and wasn't thinking straight.....I was weighing out my solids into the same beaker: sodium
fluoride, SDS and sodium deoxycholate however I had rinsed my beaker first, so the bottom was wet...which was probably a no no. Anyway, I weighed out
the NaF, then the SDS (C12H25NaO4S) and when I added the sodium deoxycholate (C24H39NaO4) and unanticipated reaction occurred producing a sharp nasty
smell that took my breath away and turned some of the solids yellow. My chemistry is rusty. Can anyone enlighten me as to what reaction would have
occurred? I'm hoping I didn't produce HF....Pasrules - 10-2-2015 at 21:05
No hydrofluoric acid would have been produced, if you want to be sure take a look at your glassware for any marks/roughness.
Also what solvent where you going to make your buffer in if water caused an "unanticipated reaction" or was this a first trial?
[Edited on 11-2-2015 by Pasrules]klowen - 10-2-2015 at 21:14
Making RIPA buffer. I would normally weigh out my solids separately and add them straight to 100 ml of MQ but I took a bad shortcut and weighed them
all out together. Then voila, punched in the lung with a gas. Guess it may have been SO3? Really do want to know what it would have been....Pasrules - 10-2-2015 at 21:46
Where did you find that recipe for the RIPA buffer?
A quick search of my local suppliers gives the composition as:
25mM Tris-HCl (pH 7.6)
150mM NaCl
1% NP-40
1% sodium deoxycholate
0.1% SDS
I understand this doesn't answer your question but I found it strange that you used NaF which is why i didn't even realise it was RIPA buffer in the
first place.klowen - 10-2-2015 at 22:10
On the Lifesciences site - it's a GeneTex protocolPasrules - 10-2-2015 at 22:29
I don't think i can provide any light onto what you created but here is the reference you mentioned for anybody else interested.
producing a sharp nasty smell that took my breath away
Sounds like airborne SDS to me. phlogiston - 11-2-2015 at 14:09
The fluoride serves as a protease inhibitor. It would not be in the recipe for the buffer itself, it is commonly added in addition to the components
of the buffer, typically along with a couple of other protease inhibitors (each of which is effective against a class of proteases).
+1 on the airborne SDS. Handle it in a fume hood, it is an extremely irritating and dusty powder.klowen - 11-2-2015 at 17:58
I had a dust mask on so it wasn't the airborne SDS.. it was definitely a vapour or gas of some sort. And the reaction only occurred in the middle of
the beaker, so basically directly between the SDS and the Na deoxycholate. And the reaction only occurred upon addition of deoxycholate. And as the
reaction caused a yellowing of the solids on top, I'm guessing the reaction was exothermic.... but am no closer to figuring out what actually
occurred. And, yes, I definitely made sure I was adding the correct solidsblogfast25 - 12-2-2015 at 09:41
Try and repeat the incident in controlled conditions and observe carefully.
