Sciencemadness Discussion Board

uses And Reactions Of Fluorescien?

SimpleChemist-238 - 28-9-2014 at 05:59

Last year I briefly experimented with fluorescien. Adding it to water and the usual. I also used it as a PH indicator. This week I started another extraction.

I placed two high lighter fluid containers in 200ml of water. Then I stored with heat and aloud the fluids to dissolve. To make sure I tested with a UV light and made sure it glowed.

With a new source of fluorescein I wanted to ask what uses or reactions I could perform with this interesting material.
Thanks.

gdflp - 28-9-2014 at 06:23

You could brominate it to make eosin, or iodinate to make erythrosin. The procedure is available in Vogel's Organic Chemistry 3rd edition in the forum library.

SimpleChemist-238 - 28-9-2014 at 07:02

can you provide a link, I can not find it in the forum library?

Texium - 28-9-2014 at 07:55

It's there. Just look for Arthur Vogel as the author. Practical Organic Chemistry is the title.

gdflp - 28-9-2014 at 08:01

Here try this

SimpleChemist-238 - 28-9-2014 at 08:51

where is it in the article, its 800 pages? BTW the library is great. Thanks for the link!

[Edited on 28-9-2014 by SimpleChemist-238]

Brain&Force - 28-9-2014 at 09:04

Highlighters contain pyranine, not fluorescein.

SimpleChemist-238 - 28-9-2014 at 09:10

thanks, I actual heard that but could not quickly confirm it. So would a reaction of fluorescien work with pyridine. I know that by adding a acid it can turn blue. I have a bottle of it stored my lab.

Texium - 28-9-2014 at 09:12

Some highlighters contain fluorescein. If it boils down to a dark reddish-brown substance, it's most likely fluorescein. I know we already had a discussion about this somewhere months ago, but I can't find it...

SimpleChemist-238 - 28-9-2014 at 09:15

I still have mine in solution so I guess I will boil it down

gdflp - 28-9-2014 at 09:26

Ctrl-F might be useful for that. It's page 985. BTW If you don't have any experience in chemistry, I would recommend not even trying to make bromine.

Brain&Force - 28-9-2014 at 09:32

I believe pyranine is more sensitive to pH changes, but I could be wrong.

SimpleChemist-238 - 28-9-2014 at 09:36

I have 2 years of experience. I have made bromine and invented a great way to make nitric acid. Bromine is easy to make but I do not have any sodium bromide at the moment. Distillation proves difficult. I have never dried bromine before but I have a fume hood.

SimpleChemist-238 - 28-9-2014 at 09:53

well it looks easy. I wonder if it would work with pyranine. Just add bromine to it and alcohol filter and wash wit alcohol. thank you for the source! Though it would make TetrabromoPyranine or something similar.

[Edited on 28-9-2014 by SimpleChemist-238]