benjyman - 31-5-2004 at 11:26
Hi I'm looking for references or places to look for information pertaining to the Idenification process of testosterone and it's various
esters.
Any help would be appreciated!
Cheers
Benjyman
unionised - 31-5-2004 at 15:08
GC/MS works quite well. I guess that immunoassay would do the trick too.
Well, that's how the professionals would do it. I think that, if all you want is a "how do they do this?" guide, then any textbook on
organic analysis would help you.
I seem to remember that there are a couple of compounds called Girrard's reagents that are used in extracting ketosteroids.
In terms of home labs, I think that you are down to measuring melting points (153-155C); specific optical rotation (115.6 deg); or bioassay.
If all else fails, you can inject it and see what happens; IIRC one of the effects is shrinkage of the testes.
Unfortunately, some things really are not in the remit of most home experimenters.
[Edited on 31-5-2004 by unionised]
benjyman - 1-6-2004 at 06:25
Thanks for the quick reply.
How about using tlc with rough Rf values for a given solvent.
did they use ms/gc in the 40/50s?
or xray crystalography?
what I mean is they must have Identified it low tech, this may be reproducable at home?
unionised - 1-6-2004 at 14:52
Yes, they used low tech. They used bioassay- feed it (or inject it into) rats.
I don't think that you will repeat their data.
Blind Angel - 2-6-2004 at 09:47
Biologicly it's produced like this
(attachement in next post)
[Edited on 2-6-2004 by Blind Angel]
Blind Angel - 2-6-2004 at 09:58
ASTII!!!!
Sorry, i just saw that it was only givind androsterone and not testosterone, can be interesting anyway.
[Edited on 2-6-2004 by Blind Angel]
