Cyanocobalamin - 27-1-2012 at 07:59
I am new to this thread~
So I was planning a synthesis to achieve pyruvic acid...
I have tiny amounts of Lactic acid, but not enough for experiementation and more than 2 attempts, so i started with Malic acid instead
I oxidized malic acid with acidified potassium permanganate, which upon addition the purple color changes from purple to brown, then it fades to a
colorless solution. I added sufficient amount of permanganate to oxidize malic acid to oxaloacetic acid. Some CO2 comes out of solution. But when I
took some of the resulting solution and add it to CaCl2 solution, there's no precipitate! Aren't calcium pyruvate insoluble? if not, how should I
separate it from the solution?
So for easy separation, I used Chromic acid to oxidize Malic acid as a second experiment, the color of solution changes from orange to brown, then
purple over a couple of minutes...but when I add a portion of this to CaCl2 solution again, no precipitates
how should I test the presence of pyruvate? and I should I prepare it? I don't have distilling apparatus yet so I can't afford the tartaric acid
route...
phlogiston - 27-1-2012 at 08:47
Are you sure about the solubility?
googling for you:
https://www.spectrumchemical.com/MSDS/C3461.PDF
says 'partially soluble in cold water'.
Perhaps you need to concentrate it a bit?
BTW it is apparantly available as a supplement, but perhaps you are just doing it for the fun.
Cyanocobalamin - 27-1-2012 at 08:50
so if it's not quite soluble how should I separate it from the solution?
thanks anyway for the information 