Steam - 2-4-2024 at 13:05
Does anyone have any recomendations for third party labs for hire to run human cancer cell viability tests? I have contacted a couple US based
companies such as Charles river and Pacifc Biolabs, but both might be a bit outside of my budget and require huge upfront payments of roughly $20,000
or so (not counting reagent acquisition costs).
I basically want to take wild cells and cancer cells (Human LOX-IMVI melanoma cells ), treat expose to several different compounds in DMSO, and then
get a fractional viability counts (as normalized to DMSO). I think this can be done for under $10,000 (not including materials/equipment costs).
Perhaps someone here can give me a reality check. I am feeling a bit dishearted by the first two quotes.
clearly_not_atara - 3-4-2024 at 10:17
I'm pretty sure you will just kill the cells with DMSO... but anyway you should be able to run a clonogenic assay yourself, particularly if you think
you're going to cure cancer
Texium - 3-4-2024 at 10:34
It's actually quite typical to use DMSO as the vehicle in cell toxicity assays, which generally include control populations with and without exposure
to DMSO alone.
Precipitates - 3-4-2024 at 19:29
Definitely, at low concentrations you're totally fine:
My results from a cell culture course I did a few years ago (1A2 cell line).
Anthracene - 11-6-2024 at 14:38
Do you have to do it by a company? If not search for university labs that do this kind of research, mail the PI and offer to supply the materials and
maybe a little stipend for a B. Sc. or M. Sc. thesis project.
Consumables are quite pricey, but the cost of labor and labspace is what makes contract research so expensive.
So if you can find a university that has the labspace and students that are willing to work for free that is the way to go.
Don't think of it as exploiting young enthusiasts, I am very grateful for the year of unpaid work I was allowed to do for my thesis project.
Considering DMSO, yes, it can absolutely be used in cell culture. Just make sure to include a control with DMSO and nothing else.
Metacelsus - 12-6-2024 at 07:40
Yeah, that's a typical price if you want to outsource it. My girlfriend works at a cancer lab at the Broad Institute and they make a lot of money by
selling multiplexed cancer viability assays to pharma companies. (They can test several hundred cell lines at once.)