Difference between revisions of "Pipette"
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==Types== | ==Types== | ||
| − | ===Air displacement micropipette=== | + | ===[[Air displacement micropipette]]=== |
Piston-driven air displacement adjustable micropipette that deliver a measured volume of liquid. Come in several sizes, from 0.1 µl to 1000 µl (1 ml). These pipettes require disposable tips that come in contact with the fluid. | Piston-driven air displacement adjustable micropipette that deliver a measured volume of liquid. Come in several sizes, from 0.1 µl to 1000 µl (1 ml). These pipettes require disposable tips that come in contact with the fluid. | ||
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Used in microinjections and patch clampings. | Used in microinjections and patch clampings. | ||
| − | ===Graduated pipettes=== | + | ===[[Graduated pipettes]]=== |
Graduated pipettes are a type of macropipette consisting of a long tube with a series of graduations, like those found on a graduated cylinder or [[burette]], to indicate different calibrated volumes. | Graduated pipettes are a type of macropipette consisting of a long tube with a series of graduations, like those found on a graduated cylinder or [[burette]], to indicate different calibrated volumes. | ||
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===[[Pasteur pipette]]=== | ===[[Pasteur pipette]]=== | ||
Pasteur pipettes are plastic or glass pipettes used to transfer small amounts of liquids, but lack gradations of any kind. The liquid is pipetted using a rubber bulb, which is separate from the pipette body. | Pasteur pipettes are plastic or glass pipettes used to transfer small amounts of liquids, but lack gradations of any kind. The liquid is pipetted using a rubber bulb, which is separate from the pipette body. | ||
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| + | Transfer or Beral pipettes are a type of Pasteur pipette, made entirely of plastic, with the bulb being part of the pipette body. | ||
===Positive displacement pipette=== | ===Positive displacement pipette=== | ||
Similar to air displacement pipettes, but the difference being the disposable tip is a plastic microsyringe, composed of a plunger which directly displaces the liquid. Less commonly used and are used to avoid contamination and for volatile or viscous substances at small volumes, such as DNA. | Similar to air displacement pipettes, but the difference being the disposable tip is a plastic microsyringe, composed of a plunger which directly displaces the liquid. Less commonly used and are used to avoid contamination and for volatile or viscous substances at small volumes, such as DNA. | ||
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===[[Volumetric pipettes]]=== | ===[[Volumetric pipettes]]=== | ||
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Can be purchased from lab suppliers. | Can be purchased from lab suppliers. | ||
| − | Pasteur and | + | Pasteur and glass pipettes can be found in many electronic cigarette stores. |
==Handling and safety== | ==Handling and safety== | ||
Revision as of 21:34, 3 March 2018
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A pipette or pipet is a laboratory tool commonly used in chemistry, biology and medicine to transport a measured volume of liquid, often as a media dispenser.
Contents
Types
Air displacement micropipette
Piston-driven air displacement adjustable micropipette that deliver a measured volume of liquid. Come in several sizes, from 0.1 µl to 1000 µl (1 ml). These pipettes require disposable tips that come in contact with the fluid.
Glass micropipette
Used in microinjections and patch clampings.
Graduated pipettes
Graduated pipettes are a type of macropipette consisting of a long tube with a series of graduations, like those found on a graduated cylinder or burette, to indicate different calibrated volumes.
Ostwald–Folin pipette
A special pipette used in measuring viscous fluid such as whole blood.
Pasteur pipette
Pasteur pipettes are plastic or glass pipettes used to transfer small amounts of liquids, but lack gradations of any kind. The liquid is pipetted using a rubber bulb, which is separate from the pipette body.
Transfer or Beral pipettes are a type of Pasteur pipette, made entirely of plastic, with the bulb being part of the pipette body.
Positive displacement pipette
Similar to air displacement pipettes, but the difference being the disposable tip is a plastic microsyringe, composed of a plunger which directly displaces the liquid. Less commonly used and are used to avoid contamination and for volatile or viscous substances at small volumes, such as DNA.
Volumetric pipettes
Volumetric pipettes, also known as bulb pipettes, consist of a glass tube with a large bulb in the middle, with a single graduation mark above the bulb, being calibrated for a single volume (similar to a volumetric flask). his type of pipette allows the user to measure a volume of solution extremely precisely.
Availability
Can be purchased from lab suppliers.
Pasteur and glass pipettes can be found in many electronic cigarette stores.
Handling and safety
It's considered a bad lab practice to draw liquid inside the pipette using your mouth suction, even if the liquid/solution is harmless.
