Difference between revisions of "Fehling's solution"
(→Availability) |
|||
| Line 113: | Line 113: | ||
}} | }} | ||
}} | }} | ||
| − | '''Fehling's solution''' is the name given to a chemical reagent, primarily used to differentiate between water-soluble carbohydrate and ketone functional groups. It can also be used as a test for reducing sugars and non reducing sugars. This reagent is prepared from two solutions, a basic solution of Rochelle Salt and a solution of Copper Sulfate. | + | '''Fehling's solution''' is the name given to a chemical reagent, primarily used to differentiate between water-soluble carbohydrate and ketone functional groups. It can also be used as a test for reducing sugars and non reducing sugars. This reagent is prepared from two solutions, a basic solution of Rochelle Salt and a solution of [[Copper Sulfate]]. |
==Properties== | ==Properties== | ||
Revision as of 19:02, 7 February 2016
 |
This article is a stub. Please help Sciencemadness Wiki by expanding it, adding pictures, and improving existing text.
|
| Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa). | |
| Infobox references | |
Fehling's solution is the name given to a chemical reagent, primarily used to differentiate between water-soluble carbohydrate and ketone functional groups. It can also be used as a test for reducing sugars and non reducing sugars. This reagent is prepared from two solutions, a basic solution of Rochelle Salt and a solution of Copper Sulfate.
Contents
Properties
Chemical
Physical
Availability
Reagent "A" and Reagent "B" are available from Sigma Aldrich.
Preparation
- Fehling's "A" uses 7 g CuSO4.5H2O dissolved in distilled water containing 2 drops of dilute sulfuric acid.
- Fehling's "B" uses 35g of potassium tartrate and 12g of NaOH in 100 ml of distilled water.
These two solutions should be stoppered and stored until needed.
For the test: Mix 15 ml of solution-"A" with 15 ml of solution-"B" Add 2 ml of this mixture to an empty test tube. Add 3 drops of the compound to be tested to the tube. Place the tube in a water-bath at 60° C.
A positive test is indicated by a green suspension and a red precipitate
Projects
Handling
Safety
Storage
Solution A and B should be stored separately in tightly stoppered bottles. They are stable under normal temperatures and pressures.
Disposal
References
Preparation from UWI at Mona, Jamaica: [[1]]
