View source for Rhodamine B

{{Chembox
| Name = Rhodamine B
| Reference =
| IUPACName = 9-(2-Carboxyphenyl)-6-(diethylamino)-N,N-diethyl-3H-xanthen-3-iminium chloride
| PIN =
| SystematicName =
| OtherNames = Basic Violet 10<br>Brilliant Pink B<br>C.I. 45170<br>C.I. Pigment Violet 1<br>Rhodamine 610
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| Section1 = {{Chembox Identifiers
| 3DMet = 
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| Section2 = {{Chembox Properties
| AtmosphericOHRateConstant = 
| Appearance = Red to violet powder
| BoilingPt = 
| BoilingPtC = 
| BoilingPt_ref = 
| BoilingPt_notes = Decomposes
| Density = 
| Formula = C<sub>28</sub>H<sub>31</sub>ClN<sub>2</sub>O<sub>3</sub>
| HenryConstant = 
| LogP = 
| MolarMass = 479.02 g/mol
| MeltingPt = 
| MeltingPtC = 210-211
| MeltingPt_ref = 
| MeltingPt_notes = (decomposition)
| Odor = Odorless
| pKa = 
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| Solubility = 8 to 15 g/L (20 °C)
| SolubleOther = Soluble in [[methanol]], [[ethanol]]
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| VaporPressure = ~0 mmHg
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| Section6 = {{Chembox Hazards
| AutoignitionPt = 
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| ExternalMSDS = [https://www.docdroid.net/XnXJmbV/rhodamine-b-sa-pdf Sigma-Aldrich]
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| MainHazards = Irritant
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| Section7 = {{Chembox Related
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| OtherCompounds = [[Fluorescein]]
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}}
'''Rhodamine B''' is a chemical compound and a dye. It is often used as a tracer dye within water to determine the rate and direction of flow and transport, or more commonly as staining fluorescent dye in biology.

==Properties==
===Chemical===
Rhodamine B can exist in equilibrium between two forms: an "open"/fluorescent form and a "closed"/nonfluorescent spirolactone form. The "open" form dominates in acidic condition while the "closed" form is colorless in basic condition.

Rhodamine B is used in biology as a staining fluorescent dye, sometimes in combination with auramine O, as the auramine-rhodamine stain to demonstrate acid-fast organisms, notably Mycobacterium

===Physical===
Rhodamine B is a reddish solid, soluble in water and alcohol.

==Availability==
Rhodamine B is sold by chemical suppliers.

==Preparation==
Rhodamine B can be prepared by reacting [[phthalic anhydride]] with m-diethylaminophenol.

==Projects==
*Stain dye
*Fluorescent dye
*Make Opera Rose

==Handling==
===Safety===
Rhodamine B is harmful if ingested in large amounts. In California, rhodamine B is suspected to be carcinogenic and thus products containing it must contain a warning on its label. Rhodamine B is notorious for its staining properties, even minute traces will stain.

===Storage===
In closed airtight bottles.

===Disposal===
Should be strongly diluted with water and poured down the drain.

==References==
<references/>
===Relevant Sciencemadness threads===
*[https://www.sciencemadness.org/whisper/viewthread.php?tid=22961 Rhodamine B]

[[Category:Chemical compounds]]
[[Category:Organic compounds]]
[[Category:Aromatic compounds]]
[[Category:Carboxylic acids]]
[[Category:Dyes]]
[[Category:Fluorescent dyes]]