Probably none at all, as lysozyme is quite stable. Just elute with more buffer, but of course expect the protein to come out later as air won't carry
the protein forward in the column.
However, being a protein, air exposure may cause precipitation on that very gel filtration column. To remove the air, wash the column with 50% MeOH,
about a litre. Then remove the precipitated protein (which is potentially stuck to the column) with 6M guanidinium hydrochlorid. Your column should
then be fully regenerated....
Never Stop to Begin, and Never Begin to Stop...
Tolerance is good. But not with the intolerant! (Wilhelm Busch)
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