Positon
Harmless
Posts: 8
Registered: 3-9-2006
Member Is Offline
Mood: No Mood
|
|
Dimethoxytrityl
Anyone can describe me the synthesis of Dimethoxytrityl ?
|
|
|
Ramiel
Vicious like a ferret
Posts: 484
Registered: 19-8-2002
Location: Room at the Back, Australia
Member Is Offline
Mood: Semi-demented
|
|
A little more info would be helpful here. Do you want to know syntheses for academic purposes, or perhaps you want to make some yourself, or are you
interested in AA syntheses using (4,4)-DMT?
We order all of our primers in for lab work from Sigma Aldrich I think (I could check if you'd like) which is generally your best bet if your aim is
to do some molecular biology.
Otherwise, you might find synthesis a little hard. What are you starting with, what kind of equipment do you have?
Caveat Orator
|
|
|
solo
International Hazard
Posts: 3975
Registered: 9-12-2002
Location: Estados Unidos de La Republica Mexicana
Member Is Offline
Mood: ....getting old and drowning in a sea of knowledge
|
|
The Dimethoxytrityl Resin Product Anchored Sequential Synthesis Method (DMT PASS): A Conceptually Novel Approach to Oligonucleotide
Synthesis
Jeffery C. Mihaichuk, T. Brian Hurley, Kurt E. Vagle, Randall S. Smith, John A. Yegge, Gretchen M. Pratt, Christopher J. Tompkins, David P.
Sebesta,* and Wolfgang A. Pieken
Organic Process Research & Development 2000, 4, 214-224
Abstract
Presented is a conceptually novel approach to oligonucleotide
synthesis referred to herein as dimethoxytrityl resin product
anchored sequential synthesis (DMT PASS). The DMT PASS
process is characterized by the reaction of a 3¢-protected
nucleoside or oligonucleotide with an excess of a nucleotide 3¢-
phosphoramite or H-phosphonate which is bound to a dimethoxytrityl
functionalized polystyrene resin. As a result, successfully
coupled oligonucleotide product is then attached to the solid
support, allowing for removal and potential recovery of starting
materials. The protected oligonucleotide product is then released
and subjected to an aqueous/organic extractive purification,
which serves to remove monomeric impurities. The PASS
process is anticipated to provide for the cost-effective manufacture
of oligonucleotides on a scale which would allow for
clinical development and pharmaceutical product commercialization.
Herein we describe some aspects of our process
development progress and discuss preliminary applications of
the process to the synthesis of short oligodeoxyribonucleotide
sequences, as well as a number of challenges confronting the
PASS development effort.
Attachment: The Dimethoxytrityl Resin Product Anchored Sequential Synthesis Method.pdf (149kB)
This file has been downloaded 946 times
It's better to die on your feet, than live on your knees....Emiliano Zapata.
|
|
|
Positon
Harmless
Posts: 8
Registered: 3-9-2006
Member Is Offline
Mood: No Mood
|
|
Thanks a lot solo , thats exactly what i'm looking for .  great !
Yes ramiel i want to make dimethoxytrityl myself , but i must know what i need first ... 
|
|
|
![[*]](images/xpblue/default_icon.gif){kind=icon}
