Tao
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Providing oxygen to an aerobic culture in a flask
What would the best way to provide extra oxygen to an aerobic bacteria in a flask? Two hole stopper, one with an aerator? Just stick the aerator in
it? (A simple fish pump, even) What's the most effective way of doing this?
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Nicodem
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Thread Moved 10-6-2013 at 07:06 |
phlogiston
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The standard way to do it is to use a large erlenmeyer, say 5x to 10x larger than your culture volume and to shake it around (most incubators for
bacterial cultures have a large plate mounted inside that will shake the flasks). Baffled flasks exist to get even more effective aeration.
![](http://catalog2.corning.com/Lifesciences/images/labware_small/4450_B_sm.jpg)
If that is not good enough for your purposes, you might try bubbling air through the culture with an aquarium pump. Run the air through a filter to
sterilize it.
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"If a rocket goes up, who cares where it comes down, that's not my concern said Wernher von Braun" - Tom Lehrer
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