chemrox
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acidic extracts from soil
I want to find a system for processing large numbers of soil samples analyzing for oxalic and possibly other low MW acids. This is going to be a
large, two year project involving soils from many different environments. Since I have no support for it yet and possibly never, I have to do
everything as cheaply as possible. The first issue is extraction; what solvent to use? The second is what detection method? A quantitative or
semi-quantitative one is needed. I have found some colorimetric methods for oxalic but the reagents aren't cheap.
[Edited on 13-11-2012 by chemrox]
"When you let the dumbasses vote you end up with populism followed by autocracy and getting back is a bitch." Plato (sort of)
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bfesser
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What relevant papers have you dug up from the literature? What is the goal of your analysis?
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chemrox
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Good questions-I have one good research paper and a process from an old set of texts on colorimetric methods by Snell & Snell. I will post the
paper tomorrow when I have my other laptop in front of me. The other part of the study is GIS for the fieldwork and I'm comfortable there.
"When you let the dumbasses vote you end up with populism followed by autocracy and getting back is a bitch." Plato (sort of)
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chemrox
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Here are some of the ones I found online
Attachment: Reference_11_19_2012_042804.pdf (7kB)
This file has been downloaded 1271 times
Attachment: Reference_11_19_2012_043039.pdf (4kB)
This file has been downloaded 540 times
Attachment: Reference_11_19_2012_043207.pdf (5kB)
This file has been downloaded 557 times
Attachment: Desk study on total organic carbon (TOC).pdf (95kB)
This file has been downloaded 727 times
Its part of C sequestration study I'm doing so I;m looking for a way to extract a lot of samples and estimate oxalic content in them. Locations will
be determined to within a meter and loaded into a GIS application made in arcmap or one of those. Keeps me stuck in windoze for the duration on at
least one machine.
[Edited on 19-11-2012 by chemrox]
"When you let the dumbasses vote you end up with populism followed by autocracy and getting back is a bitch." Plato (sort of)
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ssdd
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Reference
"Procedures for the extraction of LMWOAs
Moist field soil (20.0 g) was added to a 100 ml plastic
centrifuge tube, followed by the addition of 10 ml of 1.0 M KF
with pH 4.0 and shaking for 2 h. Soil suspensions were added
with 30 ml of 0.1 M NaOH, and shaken again in a thermostat
reciprocating shaker at 4 ± 0.5 ̊C. After a given time, the
suspension was centrifuged at 10000 rpm for 30 min. The
obtained supernatant was acidified with 0.1 – 5.0 M HCl to
precipitate humic acids, followed by filtration. The filtrates
were extracted three times for the LMWOAs with 10 ml ethyl
acetate. Then, a solution containing the LMWOAs was
obtained by evaporation of the solvent in a rotary evaporator at
40 ̊C and redissolution of the residue in 1 ml double-distilled
water. All of the samples were analyzed in triplicate, unless
otherwise stated."
I work with OAs from soil fungi, I might be able to check at work for what method we use or get a paper from a non-free journal/db
ssdd
All that glitters may not be gold, but at least it contains free electrons.
-- John Desmond Baernal
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