calamus
Harmless
Posts: 16
Registered: 11-5-2008
Member Is Offline
Mood: No Mood
|
|
requesting a calculation issue
i hope someone could explain me when I'm analyzing a plant material for carbohydrates , lipids , phenolic compounds , flavonoids or protien "total"
how i express these data as total carbohydrates was 20 mg/1g DW (dry weight) and some times FW (fresh weight)
|
|
psychokinetic
National Hazard
Posts: 558
Registered: 30-8-2009
Location: Nouveau Sheepelande.
Member Is Offline
Mood: Constantly missing equilibrium
|
|
You just did.
20mg/g DW or
20mg/g FW or
20mg/g average concentration over FW+DW etc.
As long as you state what FW and DW mean, and how they are decided upon.
“If Edison had a needle to find in a haystack, he would proceed at once with the diligence of the bee to examine straw after straw until he found
the object of his search.
I was a sorry witness of such doings, knowing that a little theory and calculation would have saved him ninety per cent of his labor.”
-Tesla
|
|
calamus
Harmless
Posts: 16
Registered: 11-5-2008
Member Is Offline
Mood: No Mood
|
|
hi psychokinetic ,
thanks for replying yes i said it is represented as FW or DW
but i wanna understand how we reach these values from say 1 g of plant material fresh or dry and extracting it by a solvent say 20 ml and then
measuring it on spectrophotometer and obtaining concentration from standard curve we prepared
then expressing the data as values mentioned 20 mg/g
|
|
psychokinetic
National Hazard
Posts: 558
Registered: 30-8-2009
Location: Nouveau Sheepelande.
Member Is Offline
Mood: Constantly missing equilibrium
|
|
Oh, I think I see what you mean. Sorry!
You want to show how 1g/20ml of test solution comes up with the end result of 20mg/g x?
Hmm. Unfortunately I'm not sure on that one, though it may be easier to figure out if I was actually doing it
“If Edison had a needle to find in a haystack, he would proceed at once with the diligence of the bee to examine straw after straw until he found
the object of his search.
I was a sorry witness of such doings, knowing that a little theory and calculation would have saved him ninety per cent of his labor.”
-Tesla
|
|
calamus
Harmless
Posts: 16
Registered: 11-5-2008
Member Is Offline
Mood: No Mood
|
|
thanks psychokinetic
i'll will try to resolve that
|
|