The Kastle-Meyer test is a test used in forensics to detect blood, whereupon blood is swabbed, the Phenolphthalein solution is added, followed by the
addition of Hydrogen Peroxide. The heme- group in blood catalyses the decomposition of H.P.
The clue of this test is that the Phenolphthalein used is not of the "normal" kind, but has been pre- dissolved in alkaline solution, and reduced to a
colorless species using zinc powder.
My question:
Has anyone got any experience in doing this? I tried it once, but my results were not any good. Is there any other way of reducing Phenolphthalein to
its colorless species?
Could not find any information elsewhere...
http://www.ncsu.edu/kenanfellows/2002/pligon/forensics/labs/...
*Luminol: 1.0 g luminal + .5 g Na2CO3 dissolved in 100 ml of distilled water, then add 0.7 g sodium perborate or 0.9 g potassium iodate.
** Kastle- Meyer reagent - This is a reduced phenolphthalein solution. KOH, phenolphthalaein and Zinc. After dissolved add ethanol to 500 ml store in
a brown bottle in the refrigerator.