Steam
Hazard to Others
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Registered: 25-3-2014
Location: Minnesota
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Mood: Triple Point
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Third Party Labs for Hire (Need Reality Check)
Does anyone have any recomendations for third party labs for hire to run human cancer cell viability tests? I have contacted a couple US based
companies such as Charles river and Pacifc Biolabs, but both might be a bit outside of my budget and require huge upfront payments of roughly $20,000
or so (not counting reagent acquisition costs).
I basically want to take wild cells and cancer cells (Human LOX-IMVI melanoma cells ), treat expose to several different compounds in DMSO, and then
get a fractional viability counts (as normalized to DMSO). I think this can be done for under $10,000 (not including materials/equipment costs).
Perhaps someone here can give me a reality check. I am feeling a bit dishearted by the first two quotes.
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clearly_not_atara
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I'm pretty sure you will just kill the cells with DMSO... but anyway you should be able to run a clonogenic assay yourself, particularly if you think
you're going to cure cancer
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Texium
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It's actually quite typical to use DMSO as the vehicle in cell toxicity assays, which generally include control populations with and without exposure
to DMSO alone.
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Precipitates
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Definitely, at low concentrations you're totally fine:
My results from a cell culture course I did a few years ago (1A2 cell line).
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Anthracene
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Do you have to do it by a company? If not search for university labs that do this kind of research, mail the PI and offer to supply the materials and
maybe a little stipend for a B. Sc. or M. Sc. thesis project.
Consumables are quite pricey, but the cost of labor and labspace is what makes contract research so expensive.
So if you can find a university that has the labspace and students that are willing to work for free that is the way to go.
Don't think of it as exploiting young enthusiasts, I am very grateful for the year of unpaid work I was allowed to do for my thesis project.
Considering DMSO, yes, it can absolutely be used in cell culture. Just make sure to include a control with DMSO and nothing else.
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Metacelsus
International Hazard
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Registered: 26-12-2012
Location: Boston, MA
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Mood: Double, double, toil and trouble
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Yeah, that's a typical price if you want to outsource it. My girlfriend works at a cancer lab at the Broad Institute and they make a lot of money by
selling multiplexed cancer viability assays to pharma companies. (They can test several hundred cell lines at once.)
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