vegafelisa
Harmless
Posts: 3
Registered: 20-8-2019
Member Is Offline
|
|
Extraction microrna From Humans Plasma
Hi. My question is: what is the best way for extraction microrna from humans plasma? Whether trizol is suitable, or i will should use commercial kits?
Thanks
|
|
|
Metacelsus
International Hazard
Posts: 2539
Registered: 26-12-2012
Location: Boston, MA
Member Is Offline
Mood: Double, double, toil and trouble
|
|
Trizol should work, but under some conditions low GC miRNAs could be lost. Make sure to use appropriate controls. See the attached references.
Attachment: PIIS1097276512004819.pdf (350kB)
This file has been downloaded 565 times
Attachment: s12864-015-1299-5.pdf (666kB)
This file has been downloaded 547 times
Also this thread should probably be moved to the Biochemistry subforum.
[Edited on 2019-10-21 by Metacelsus]
|
|
|
Texium
|
Thread Moved
21-10-2019 at 05:54 |
vegafelisa
Harmless
Posts: 3
Registered: 20-8-2019
Member Is Offline
|
|
Thank you so much, really helpful these two attachments. I will keep your suggestion in mind next time I have any questions.
|
|
|
![[*]](images/xpblue/default_icon.gif){kind=icon}
