Sulaiman
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Is an automatic transfer pipette useful - which one ?
I have un-calibrated disposable 1ml and 2ml plastic pipettes which I find so useful that I buy by the 100's and re-order before I finish existing
stock.
I have a 10ml roughly calibrated borosilicate pipette with rubber bulb, also very useful.
Occasionally I use a 2 or 10 ml borosilicate syringe for transfers.
For accurate measurements I use burettes or weight
(or volumetric flasks of 10, 25, 50, 250 or 500 ml capacity).
It would be convenient if I had a calibrated pipette for tasks such as taking a sample of known volume, of a liquid for analysis,
or adding a known volume of a reactant.
The actual volume is not important to me, around 1 ml seems suitable, but repeatability is critical.
Would a pipettor such as this be suitable ?
https://www.ebay.co.uk/itm/100ul-500ul-Lab-Micro-Transfer-Pi...
(I suspect not long-term repeatable)
Any recommendations for a pipete or similar to transfer a reliably known volume (preferably better than +/-1%) of liquid every time,
preferably compatible with all known liquids ![:D](./images/smilies/biggrin.gif)
and cheap ![:P](./images/smilies/tongue.gif)
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Herr Haber
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I have a couple of automatic ones, set to a precise volume and another one where I can set the volume.
Truth is... I never use them. To much of a hassle imo but maybe I'm wrong.
I'd rather weight, cylinder and use regular pipettes for the last missing ml.
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Vanry
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I have no use for those in my home lab, but I use some at work, so, don't expect cheappy thing in this post ![:D](./images/smilies/biggrin.gif)
all the eppendorf ones are precise effective and ergonomic. Good stuff. But pretty expensive when bought brand new.
Even more expensive you got the electronic handystep from brand. But it's amazing and LARGELY worth it's price. Precise, multi-product and with a
repeatability beyond the sky... For taking a precisely known volume of liquid in a quick time, I think it's the best. And you can do 100 pippeting/day
without being tired ![:)](./images/smilies/smile.gif)
Hope this can help
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Sulaiman
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![:D](./images/smilies/biggrin.gif) ![:D](./images/smilies/biggrin.gif) ![:D](./images/smilies/biggrin.gif) ![:D](./images/smilies/biggrin.gif) ![:D](./images/smilies/biggrin.gif) ![:D](./images/smilies/biggrin.gif) ![:D](./images/smilies/biggrin.gif) ![:D](./images/smilies/biggrin.gif) ![:D](./images/smilies/biggrin.gif) ![:D](./images/smilies/biggrin.gif) ![:D](./images/smilies/biggrin.gif) ![:D](./images/smilies/biggrin.gif) :
![:D](./images/smilies/biggrin.gif) ![:D](./images/smilies/biggrin.gif) electronic handystep ![:D](./images/smilies/biggrin.gif) ![:D](./images/smilies/biggrin.gif)
![:D](./images/smilies/biggrin.gif) ![:D](./images/smilies/biggrin.gif) ![:D](./images/smilies/biggrin.gif) ![:D](./images/smilies/biggrin.gif) ![:D](./images/smilies/biggrin.gif) ![:D](./images/smilies/biggrin.gif) ![:D](./images/smilies/biggrin.gif) ![:D](./images/smilies/biggrin.gif) ![:D](./images/smilies/biggrin.gif) ![:D](./images/smilies/biggrin.gif) ![:D](./images/smilies/biggrin.gif) ![:D](./images/smilies/biggrin.gif) :
more than all of my other chemistry equipment costs combined ![:o](./images/smilies/shocked.gif)
I've decided that I really like my existing setup.
Thanks
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Dr.Bob
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If you can find a used Gilson Pipetteman (not a cheap rip off) for a good price, it might be worth it, they are very nice, and a 1ml one will measure
quite accurately.
They are commonly on Ebay here in the US, likely also in Europe as well used. Lots of similar ones, like Eppendorf ones and Eppendorf Repeater,
which is great for repeated actions. But glass pipettes also also quite good and can be found very cheap lately. Even the cheap 0.5, 1, and 2 ml
serological ones are pretty accurate for most work. I have tested some, and they are good within 2-5% repeatability even down to 0.10 ml or so.
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streety
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That type of pipette is the workhorse of any biology lab. Typically any bench will have four: <2ul, 2-20ul, 20-200ul and 200-1000ul. Larger sizes
exist also and multi-channel pipettes are fairly common.
They sometimes struggle with particular solvents. I always have to be quick when working with chloroform or they will drip.
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Dr.Bob
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The Eppendorf repeater is positive displacement, so it handles low bp solvents much better. I have used them with DCM, TFA, CHCl3, and others just
fine. Best to prime them once and fill and empty them a couple of times first to get them equilibrated with solvent vapor. I have scads of these in
almost every size and brand, so if people want some, I can make a deal. Pricer to ship to Europe, so likely better to find a local source. The
tips often cost more than the pipettor, but can be found on Ebay in deals sometimes. I have some of them as well, but not all types and sizes.
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morganbw
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They can be very beneficial, providing the conditions are the same.
Something as benign as a different temp. can cause problems.
I could write a chapter on this, just be aware that different conditions may cause a difference with a sample size.
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