rachel
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Dissolution of PHB (polyhydroxybutyrate or glutamate)
dear all
I am in a dilemma as to how to dissolve PHB with a mol wt of 470.000.On all the papers I came across Dichloromethane and Chloroform and acetone were
the options but none of them works until heating and overnight stirrring is required .CAn anybody help??
Thanks in advance
rachel
[Edited on 12-10-2005 by chemoleo]
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solo
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| Quote: |
.....methylene chloride, distilled water, and polyvinyl alcohol were utilized as the solvent,
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as read in..
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&a...
It's better to die on your feet, than live on your knees....Emiliano Zapata.
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rachel
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| Quote: | Originally posted by solo
| Quote: |
.....methylene chloride, distilled water, and polyvinyl alcohol were utilized as the solvent,
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Thanks for the info.but unfortuantely i had tried the above said and doesnt work.now i am looking for a solvent for sodium polyglutamate which i hope
u cud help.i tried with chloroform ,methylene chloride but none of them works.
regards
rachel
as read in..
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&a... |
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Eclectic
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Try DMSO, DMF, NMP, or cyclohexanone.
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DrP
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Will THF work? I don't know much about PHB but THF used to be the strongest solvent in our lab for high MW polymers.
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chemoleo
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For proteins, or polyglutamate etc, you might want to try 8 M guanidinium hydrochloride, or 6 M urea. These usually solubilise proteins very
effectively.
Otherwise, 1:1 acetonitrile:water (maybe with 0.1% TFA) might work too.
Possibly glycerol, this might help as well due to chelate effects.
Also, ultrasonication in your buffer/solvent of choice often helps during the dissolution process.
[Edited on 12-10-2005 by chemoleo]
Never Stop to Begin, and Never Begin to Stop...
Tolerance is good. But not with the intolerant! (Wilhelm Busch)
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poly2004peek
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try Tetramethylene sulfone
Tetramethylene sulfone
is very strong solvent.
PET can dissolved in Tetramethylene sulfone and PHB also a kind of polyester.
just try it.
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