Mildronate
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HPLC peak prediction
Does anbody know something about peak prediction in liquid chromatography?
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Ephesian
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hplc instruments are usually set up as "reversed phase" meaning non-polar stationary phase and a polar mobile phase. This means that your separation
will be based on the molecules degree of hydrophobicity. reversed phase is a very polar environment, so will have a stronger affinity for polar
molecules (hence the term like-dissolves-like) and your non-polar will elute first following will be the polar molecules in increasing polarity.
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phlogiston
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What type of solid phase are you using? Ion exchange, reverse phase, HILIC, gel filtration, affinity, something else?
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"If a rocket goes up, who cares where it comes down, that's not my concern said Wernher von Braun" - Tom Lehrer |
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Mildronate
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I use revese phase only. I find that there is some prediction software, but i realy dont belive it can work. In GC there is Kovach index and all is
simple, but in hplc?
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phlogiston
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It is more complex than in GC. This page describes a method that seems useable:
http://www.retentionprediction.org/hplc/howitworks.php
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"If a rocket goes up, who cares where it comes down, that's not my concern said Wernher von Braun" - Tom Lehrer |
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