Vmedvil
Banned for blowing margins instead of minds...
Posts: 42
Registered: 4-9-2017
Member Is Offline
Mood: No Mood
|
|
How to make Cats Glow in the Dark
Before reading this I suggest you learn, How to Make a HIV Vector .
This time you need to use the Feline Immunodeficiency Virus , F.I.V.
The Genome can be found F.I.V. Genome NBCI
Next, you find the gene for Bio-Luminescence, you can choose one of three colors Red,Green, or Blue. This will be green because this gene is from the
Japanese firefly.
Green Bio-luminesence Protein Japanese Firefly
Now we want to make the code for this Gene with a LTR 5' Primer on the End and LTR 3' on the Beginning using Viral Gene Synthesis .
Then Use the F.I.V. Virus's ENV gene long with Gag-Pol gene, this will be the machinery we use to transfer the Bio-Luminescence Gene
Put the genes into T-Cells to make a Packing Transfer Plasmid.
Transfect the Set of cells in the embryo or other cells that you want to glow in the dark green, which means either add it to the blood of the mother
or directly to the cells.
When that Mother cat gives birth her kittens will glow in the dark just like a Japanese Firefly having this gene added to the kittens.
|
|
|
SWIM
National Hazard
Posts: 970
Registered: 3-9-2017
Member Is Offline
|
|
Japanese fireflies are the souls of warriors who died in battle.
You start messing around with gene splicing them into cats and you'll end up with cats with the souls of dead Japanese warriors.
That's all we'd need: A bunch of glowing Samurai cats terrorizing the populace and then committing seppuku when their owners scold them.
It would be safer to use fluorescent jellyfish. That's what the South Koreans did, probably because they're aware of the hazards of having Japanese
warriors around.
It'd probably be better if you spliced DNA from an elephant and a pot belied pig to get cute little dwarf elephants.
DeaconicAcid's post below has made me reconsider my post.
We would all benefit from more pizza in this world, even if some of it does have a little cat hair on it.
(employees of this pizzeria are required to wash their paws after using the litter box)
[Edited on 7-9-2017 by SWIM]
|
|
|
Texium
Administrator
Posts: 4618
Registered: 11-1-2014
Location: Salt Lake City
Member Is Offline
Mood: PhD candidate!
|
|
Giving cats AIDS just to make them glow doesn't seem very nice...
|
|
|
Vmedvil
Banned for blowing margins instead of minds...
Posts: 42
Registered: 4-9-2017
Member Is Offline
Mood: No Mood
|
|
It won't die from AIDS the F.I.V. virions can not reproduce without the ENV Plasmid which is missing from the genome that is transferred that has LTR
3' and 5' around them. They can not replicate outside the Packaging Cell.
|
|
|
Metacelsus
International Hazard
Posts: 2539
Registered: 26-12-2012
Location: Boston, MA
Member Is Offline
Mood: Double, double, toil and trouble
|
|
As described, this plan would not work.
Firefly luciferase does not produce light on its own. It needs luciferin as a substrate to oxidize, so for light production, additional enzymes are
needed to produce the luciferin. The transgenic cat would also have to express those enzymes.
|
|
|
DraconicAcid
International Hazard
Posts: 4355
Registered: 1-2-2013
Location: The tiniest college campus ever....
Member Is Offline
Mood: Semi-victorious.
|
|
But samurai cats will make pizza!
https://www.youtube.com/watch?v=WZNt9p4LWeA
Please remember: "Filtrate" is not a verb.
Write up your lab reports the way your instructor wants them, not the way your ex-instructor wants them.
|
|
|
![[*]](images/xpblue/default_icon.gif){kind=icon}
