Little_Ghost_again
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stain diferential of rough ER and smooth ER?
Hi
are there any 'simple' stains to distinguish between rough endoplasmic reticulum and smooth endoplasmic reticulum? I am wanting to stay away from
fluorescence dyes if I can. I have had a good look and it dosnt look hopeful, but I was wondering if anyone had experience of dyes that differentiate
between the two?
Yes under highish mag I can look with a general reticulum/golgi app stain and see which is smooth and which is rough.
I am looking for a quick scan method if I can, so though just maybe there was stain that would do it, but as both are lipid based I doubt it.
I know there is a fluro stain but that requires using phase contrast and an adapter etc, plus 600x mag, at that magnification I might as well just use
a general stain or even iodine.
Dont ask me, I only know enough to be dangerous
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forgottenpassword
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I know nothing about this whatsoever but I came up with "the lack of ribosomes means that the smooth ER is not basophilic and usually stains poorly by
H&E."
http://en.wikipedia.org/wiki/H%26E_stain
http://medcell.med.yale.edu/histology/cell_lab/rough_endopla...
[Edited on 28-11-2014 by forgottenpassword]
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Little_Ghost_again
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I need to stain the ER (both types) and in a perfect world would have a stain that only stains RER one colour and SER another. But its isnt a perfect
world  , the annoying thing is I have a solution using fluro stains and some phase
contrast trickery. My problem is one I dont like the cost and two I dont like the faff!
But your statement that SER is not basophillic has given me an idea. I will try it out in the morning and see what happens  . its at times like this I wish my bloody microscope camera attachment worked!!! I
had a decent one for the Nikon camera and stupidly left it in the lab, now its dead. I might try and take it apart and fix it, or maybe buy a cheap
attachment for the camera.
I have some superb yeast cell slides I would love to photograph and post. Also I do like to photograph my slides with a decent camera, you can bring
it up on a huge monitor and really study the picture, I find it much better than just using the eye through the microscope. Modern High res camera's
take microscopy onto another level. Shame the attachments cost so much. Thanks for the links they have got me thinking of another way around it.
LG
Dont ask me, I only know enough to be dangerous
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Little_Ghost_again
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Posts: 985
Registered: 16-9-2014
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Mood: Baffled
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I managed it kind of with potassium hydroxide and eosin, The contrast isnt great but there you go.
Dont ask me, I only know enough to be dangerous
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