yogi
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Claviceps Paspali Fermentation Experiments
hello. can some1 get a hold of the claviceps.paspali.html page from the old rhodium archive? its missing from the archives ive seen. would be nice if
some1 could get the page and the photos...
thanks!
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solo
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I was only able to find this one in the Rhodium Pdf Archives, I hope it's of some assistance to your project........solo
http://rapidshare.de/files/4624190/claviceps.identification....
found some threads.....
https://www.synthetikal.com/hiveboard/tryptamine/000448301.h...
https://www.synthetikal.com/hiveboard/tryptamine/000450772.h...
https://www.synthetikal.com/hiveboard/tryptamine/000395211.h...
https://www.synthetikal.com/hiveboard/tryptamine/000452298.h...
[Edited on 2-9-2005 by solo]
It's better to die on your feet, than live on your knees....Emiliano Zapata.
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epck
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Here's another one from the Erowid Rhodium archive.
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yogi
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thanks for the replys, but i was refering tot he page where a fellow be fermented c.paspali f-240 strain (if i remember correctly) and showed pictures
of the growth stages in a fermentor....
THANKS!
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Mush
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Perhaps it is:
http://img835.imageshack.us/i/193994811post1711710257.jpg/
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madcedar
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Ergot Liquid Culture Part 1
The above post from Mush above are the pictures you want. You'll also want to read the following paper:
Attachment: Ergot Liquid Culture.part1.rar (1.4MB)
This file has been downloaded 1298 times
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madcedar
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Ergot Liquid Culture Part 2
Sorry, I had to split the file
Attachment: Ergot Liquid Culture.part2.rar (1.4MB)
This file has been downloaded 1114 times
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madcedar
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Ergot Liquid Culture Part 3
And finally part 3. You'll need winrar to put the file together again. It's a pdf about isolating and growing a liquid culture of claviceps paspali.
Success with this project would be greatly satisfying indeed. Good luck.
Attachment: Ergot Liquid Culture.part3.rar (621kB)
This file has been downloaded 1027 times
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Mush
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It's a great reference. Thanx for it!
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peach
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There are absolutely TONS of journal entries regarding the culturing of ergot.
One point I have noted, which is worth paying some attention to, is that they are using specifically selected strains of ergot, as a number of them
produce next to zero lysergides. Also, the production drifts if the culture is cloned too far from the master plate.
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Rogeryermaw
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something else worth noting about the production of ergot strains: extreme care must be taken not to allow any spore to escape your working
environment. such a breach could be devastating to local grain production and a health and environmental hazard. it's not quite like growin' 'shrooms.
ergot is dangerous.
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peach
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The gangrene effect, I believe is caused by vasoconstriction, from the lysergides in there. Which hopefully means it's temporary and not some kind of
fungal infection.
But I hadn't even thought about the first point. Farmers do have a problem with ergot. It spreads around the crops as they wave about in the wind and
that can't go out for consumption. One method of removing it is to get out there, on foot and chop down the infected areas by hand. That can't be much
fun when you're used to using harvesters with 12ft spans on them for acres and acres.
I wonder if they could find it easier in the dark by shining a UV lamp over the crops and using something that can see very tiny lights better, like
camera for a telescope. Maybe the ears would be just visible as glowing specs on the strands. That'd only work if the strain contained lysergides,
which some of them don't.
They can also remove it as the grains go through the conveyors. The ears of grain are knocked off and carried away, which can also help knock the ears
of ergot into a separate bin, since the grains of crop are a known size and can be screened.
Sandoz published some interesting papers on ergot culturing in the wild. They had big fields that looked like something out of a Sci-Fi film with huge
overhead booms on tracks, which moved up and down the fields spraying them with inoculant.
Another method they tried was to mix the 'honey dew' that drips out of the ears of ergot with a little sugar. Then they made things out of planks of
wood that go on your hands, with lots of pins sticking through the other side. The idea was to wet some of the mixture onto the boards, then get out
on foot and simply 'slap' the ears of crop between the boards. The pins would puncture the grains and inject the spores from the dew. Works as well as
the overhead booms from what I remember.
I have only heard one mention of someone ever doing this outside of a lab or for a paper, and that was someone saying something about inoculating rye
with it and then burying the jars - and that it was the rainbow group of hippies doing this. I asked if they had any details on that, no response. I
am willing to believe it's possible, but am skeptical. If it was that easy, it'd be all over the shroomery; there's a constant stream of ergot
questions on there.
[Edited on 29-10-2010 by peach]
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Random
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Is culturing ergot illegal?
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peach
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I believe it would be in a number of places - it's akin to growing psilocybe mushrooms, the spores aren't illegal, but the mushrooms are, as they
contain an illegal drug. Particularly if you have no real knowledge of the organism or interest in anything else it or the products can do, beyond
LSD.
For instance, if you were culturing just a plate of it to see it and were interested in all kinds of fungi, and had them growing, I doubt a judge
would be that bothered. Technically, that looks like you are getting ready to supply a drug, but realistically, a plate will do nothing.
If you were brewing bins full of it for 'no real reason', they'd be bothered.
[Edited on 29-10-2010 by peach]
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zed
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So, those of you out there, that have adapted your swimming pools (or Jacuzzis) for Ergot culture, be forewarned. Especially those of you, that have
Olympic Size pools.
If you are taken before a judge, you had better have a very plausible excuse.
"I was fermenting rye for whiskey, but something went terribly wrong" ????
"Sorry, I just got back from Antarctica, musta been them sub-letters."
"Sorry, I'm just the gardener, I know the pool has been stinking, but it's not my responsibility." "Call George Bush down in Texas; It's his house."
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gravityzero
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Quote: Originally posted by peach  | The gangrene effect, I believe is caused by vasoconstriction, from the lysergides in there. Which hopefully means it's temporary and not some kind of
fungal infection.
But I hadn't even thought about the first point. Farmers do have a problem with ergot. It spreads around the crops as they wave about in the wind and
that can't go out for consumption. One method of removing it is to get out there, on foot and chop down the infected areas by hand. That can't be much
fun when you're used to using harvesters with 12ft spans on them for acres and acres.
I wonder if they could find it easier in the dark by shining a UV lamp over the crops and using something that can see very tiny lights better, like
camera for a telescope. Maybe the ears would be just visible as glowing specs on the strands. That'd only work if the strain contained lysergides,
which some of them don't.
They can also remove it as the grains go through the conveyors. The ears of grain are knocked off and carried away, which can also help knock the ears
of ergot into a separate bin, since the grains of crop are a known size and can be screened.
Sandoz published some interesting papers on ergot culturing in the wild. They had big fields that looked like something out of a Sci-Fi film with huge
overhead booms on tracks, which moved up and down the fields spraying them with inoculant.
Another method they tried was to mix the 'honey dew' that drips out of the ears of ergot with a little sugar. Then they made things out of planks of
wood that go on your hands, with lots of pins sticking through the other side. The idea was to wet some of the mixture onto the boards, then get out
on foot and simply 'slap' the ears of crop between the boards. The pins would puncture the grains and inject the spores from the dew. Works as well as
the overhead booms from what I remember.
I have only heard one mention of someone ever doing this outside of a lab or for a paper, and that was someone saying something about inoculating rye
with it and then burying the jars - and that it was the rainbow group of hippies doing this. I asked if they had any details on that, no response. I
am willing to believe it's possible, but am skeptical. If it was that easy, it'd be all over the shroomery; there's a constant stream of ergot
questions on there.
[Edited on 29-10-2010 by peach] |
I hate to dig up an old article, but I did have something to add to this discussion. It would appear that as the story goes, there are many new
variations to the factor. The 3 part paper contained on this thread seems to be on the right trail. It appears that the best way to go about new
production methods is to use a good alkaloid producing strain of c. paspilla and not c. purpurea. This is from what I've found and I also hear of a
sleepy grass of KY having the goods as well. I once found a good article on a well known forum talking of Pyobp peptide coupling and from what I
gather that is the route to go.
I seriously hear of methods that require no chromatography and I won't say my sources, but from what I can tell must be legit.
So back to c. paspilla and why it is the route to go. Apparently it can be done in such a manner as to not cause gangrene or vasoconstriction. Don't
ask me how, I know not the details, but rather where to get them. Any who, think of the possibilities if that information where true?!? One could
take a visit to other popular mytocologicaly inclined forums and be well on their way.
There is particular agar that allows for good timing of the process and allows for one to transfer onto substrate. This allows for a pleasant amount
of alkaloid production, privately and somewhat safe conditions; adverse to previous discussions.
There are methods for timing transfers and for extraction of alkaloids without conventional solvents that move towards a pure and clean extraction of
specific alkaloids. Then I believe the hydrolysis or peptide coupling would commence. Again I have gathered numerous bits and pieces over quite a
few hours of research on both this and other websites.
I do not post often, but I aim to assist in information I believe to be ordained by God creator. I believe this substance has life changing
attributes. I also know in my heart there are much eaiser methods than are currently listed on your average forum.
I have been in the trenches to a degree and found places that leave me scratching my head, like, did that just happen? It appears on its face to be
tantamount to making moonshine and knowing a bit of mycology and basic chemistry. I am convinced of it. Now I'm not gonna make claims like it is
baking a cake, but it does not take an elaborate education to pull off either.
BTW the Pyobp coupling discussion I found soon disappeared from the forum I found it on. I had to locate it on the way back machine. I did retrieve
it and would be glad to share.
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solo
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......please share,....solo
It's better to die on your feet, than live on your knees....Emiliano Zapata.
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PickledPackratParalysis
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I don't know how to open a *.rar file... But I have heard of ergot before in the edible wild plants books. Apparently it's toxic!
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bfesser
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<a href="http://en.wikipedia.org/wiki/Ergot" target="_blank">Ergot</a> <img src="../scipics/_wiki.png" /> is often discussed in
relation to <a href="http://en.wikipedia.org/wiki/Lysergic_acid_diethylamide" target="_blank">LSD</a> <img src="../scipics/_wiki.png"
/> synthesis.
If you're on Windows, try <a href="http://www.rarlab.com/">WinRar</a> <img src="../scipics/_ext.png" /> (link to the official site).
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gravityzero
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That is the turning point of this whole discussion. I am at work at the moment, so I am unable to post the additional information that I have at the
moment.
To assume Ergot is toxic is DEAD on the mark. I am not suggesting the consumption of ergot alkaloids in any respect.
The reason for my newly peeked interest on this subject is I have come to understand that c. paspali has properties that make it exceedingly eaiser to
cultivate safely. From the information I have found, and I'm not sure if it is strain specific. Generally ergot alkaloid profiles change from strain
to strain. In other words, just cause you find ergot in a field, does not mean that variety would have good alkaliod production.
Now it use to be you could toss on top of that the hazmat conditions that cultivation would take on top of that. Apparently you can have the best of
both worlds. There are strains available and I know the connect, whereby good producing c. paspali can be cloned and maintained virtually anywhere,
including ones domicile. It is becomming more and more like cubensis cultivation.
If one examines the real difficulty in production, they find that beyond the needed chemistry skill set, ergot alkaliods are very hard to source. It
now appears that this difficulty will soon be over.
If you stood around and watched hoffman, back in the day. You probably would have been able to do similar things, after watching him repeatitively.
Now a days you would have to do one better than that, mainly because of precursor availability. In other words, you need to be better than hoffman.
The methods that appear to be comming to light, allow you to be back at the same starting point. I don't know that this would help the average
person, but it could not hurt.
This information does not come cheap, but if I do decide to spring for it, I would definitely share. If someone has direct workings with c. paspali
and knows this information to be incorrect as for safety goes, do share. I only aim for the truth. I am just convinced of the source of this
information.
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bfesser
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Thread Split
19-7-2013 at 13:17 |
Texium
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Thread Moved
20-12-2023 at 12:42 |
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