Steam
Hazard to Others
Posts: 238
Registered: 25-3-2014
Location: Minnesota
Member Is Offline
Mood: Triple Point
|
|
Third Party Labs for Hire (Need Reality Check)
Does anyone have any recomendations for third party labs for hire to run human cancer cell viability tests? I have contacted a couple US based
companies such as Charles river and Pacifc Biolabs, but both might be a bit outside of my budget and require huge upfront payments of roughly $20,000
or so (not counting reagent acquisition costs).
I basically want to take wild cells and cancer cells (Human LOX-IMVI melanoma cells ), treat expose to several different compounds in DMSO, and then
get a fractional viability counts (as normalized to DMSO). I think this can be done for under $10,000 (not including materials/equipment costs).
Perhaps someone here can give me a reality check. I am feeling a bit dishearted by the first two quotes.
DISCLAIMER: The information in this post is provided for general informational purposes only and may not reflect the current law in your jurisdiction.
No information contained in this post should be construed as legal advice from the individual author, nor is it intended to be a substitute for legal
counsel on any subject matter. No reader of this post should act or refrain from acting on the basis of any information included in, or accessible
through, this post without seeking the appropriate legal or other professional advice on the particular facts and circumstances at issue from a lawyer
licensed in the recipient’s state, country or other appropriate licensing jurisdiction.
|
|
|
clearly_not_atara
International Hazard
Posts: 2799
Registered: 3-11-2013
Member Is Offline
Mood: Big
|
|
I'm pretty sure you will just kill the cells with DMSO... but anyway you should be able to run a clonogenic assay yourself, particularly if you think
you're going to cure cancer
|
|
|
Texium
Administrator
Posts: 4618
Registered: 11-1-2014
Location: Salt Lake City
Member Is Offline
Mood: PhD candidate!
|
|
It's actually quite typical to use DMSO as the vehicle in cell toxicity assays, which generally include control populations with and without exposure
to DMSO alone.
|
|
|
Precipitates
Hazard to Others
Posts: 136
Registered: 4-12-2023
Location: SE Asia
Member Is Offline
Mood: Acid hungry
|
|
Definitely, at low concentrations you're totally fine:
My results from a cell culture course I did a few years ago (1A2 cell line).
|
|
|
Anthracene
Harmless
Posts: 9
Registered: 4-12-2023
Location: Stone Island
Member Is Offline
Mood: Curious
|
|
Do you have to do it by a company? If not search for university labs that do this kind of research, mail the PI and offer to supply the materials and
maybe a little stipend for a B. Sc. or M. Sc. thesis project.
Consumables are quite pricey, but the cost of labor and labspace is what makes contract research so expensive.
So if you can find a university that has the labspace and students that are willing to work for free that is the way to go.
Don't think of it as exploiting young enthusiasts, I am very grateful for the year of unpaid work I was allowed to do for my thesis project.
Considering DMSO, yes, it can absolutely be used in cell culture. Just make sure to include a control with DMSO and nothing else.
|
|
|
Metacelsus
International Hazard
Posts: 2539
Registered: 26-12-2012
Location: Boston, MA
Member Is Offline
Mood: Double, double, toil and trouble
|
|
Yeah, that's a typical price if you want to outsource it. My girlfriend works at a cancer lab at the Broad Institute and they make a lot of money by
selling multiplexed cancer viability assays to pharma companies. (They can test several hundred cell lines at once.)
|
|
|
![[*]](images/xpblue/default_icon.gif){kind=icon}
