ssdd - 28-10-2007 at 10:56
So as you have probably seen from past threads I do some minor work with plant tissue culturing.
Recently I have been hoping to work on some Callus cultures.
(This is a mass of unorganized cells that hold no properties of the tissue they came from.)
For this I was looking at using several growth inhibitors rather than promoters. I found two that may be of some use from what info I could gather,
but I was wondering if anyone here had used these or could find a bit more info regarding them.
Triiodobenzoic Acid - This is listed as a root and shoot inhibitor. (But also an auxin oddly enough) Also prevents tissues from developing polarity.
This is all I could find on it.
Paclobutrazol - This one blocks several metabolic pathways which prevents the production of several hormones. This one looks interesting but I
couldn't find much more info other than what hormones it prevents.
What ever info you can find would be great.
-ssdd
Giberellins prevented
chemrox - 28-10-2007 at 21:07
Journal of Plant Growth Regulation
Publisher Springer New York
ISSN 0721-7595 (Print) 1435-8107 (Online)
Issue Volume 10, Number 1 / December, 1991
Take a look at this article and see what you can discern from it. It appears to discuss the inhibition of giberellins formation by paclobutrazol. I
don't have access to the full text so I don't know the mechanism of the inhibition but the hormone would be giberellins as I undertand hormones in
plants. Is it also called "florigen" anymore?
here's the url (edited to show it)
http://www.springerlink.com/content/f66237m224085806/
[Edited on 28-10-2007 by chemrox]
ssdd - 29-10-2007 at 08:52
OK so I encountered a mild problem.
IAA according to several things I recently read requires refrigeration from 0 to -20 *C. I don't have the ability to store a quantity of this for long
term as I need to. So I was wondering if anyone has any ideas as to a good synthetic auxin that doesn't require such low temperatures. (room temp
preferably.)
-ssdd
feacetech - 30-10-2007 at 12:14
you dont have any sort of freezer?
ssdd - 30-10-2007 at 13:52
I do, but being I live on a college campus this is fairly difficult because the power can not be on in the rooms over breaks.
I could store it in a lab but I have already consumed a positive pressure hood and I don't want to wear my welcome in the botany lab. (Not to mention
they have a tendency to remove a lot of stuff from the freezers and fridges here because of an accident 3 years ago.)
So this is my logic in searching for a decent synthetic auxin.
-ssdd
feacetech - 30-10-2007 at 15:15
accident? do tell.
ssdd - 31-10-2007 at 04:27
A few years ago someone put a mix into a fridge that gave off a fair amount of fumes. So the fumes built up in the fridge and when the compressor
kicked on it sparked....
Regardless to say the door of the fridge was found in the wall on the other side of the lab. Now they have explosion proof fridges but are still picky
about what goes in.
-ssdd
sebinou - 23-11-2007 at 07:08
I put on my 4shared folder the article mentioned by chemrox.
It's the file Paclobutrazol.PDF
http://www.4shared.com/dir/4267407/2e605ffd/sharing.html
Hope it will be useful 
kclo4 - 23-11-2007 at 11:51
I know this bit of information isn't very useful, but i figured i'd mention it anyways.
Salicylic acid or Aspirin work as a plant growth regulator.
Oh, And also i bet its possible to extract some hormones from corn seeds.
Don't seeds have a lot of stored hormones?
..one of the draw backs would be they would be all sorts of hormones if you extracted it.
but its just an idea.
chemoleo - 23-11-2007 at 17:30
Also be aware of the similarity of auxin to tryptophan, one of the genetically encoded amino acids:
All that's required is elimination of the amino group, which could well be done via diazotation...
12AX7 - 24-11-2007 at 03:44
According to those two diagrams, the difference is a -CH(-)-NH2 unit.
But that still wouldn't be too horrible, deaminate then decarboxylate and oxidize it to the carbon-minus-one acid.
Tim
not_important - 24-11-2007 at 08:42
Ah, but the indole nucleus is a bit sensitive to some of the methods used to deaminate/decarboxylate so you must choose wisely. There are biological
paths that perform the conversion, as well as several related ones.
http://www.sheldrake.org/Articles&Papers/papers/hormone/...
http://www.patentstorm.us/patents/6180569.html
http://findarticles.com/p/articles/mi_m0DED/is_7_21/ai_70907...
http://employees.csbsju.edu/ssaupe/biol327/Lecture/hormone-a...
Eclectic - 24-11-2007 at 08:53
You should have a ready source for a few days following the great turkey feast. It's the normal waste product of tryptophan metabolism. 
[Edited on 11-24-2007 by Eclectic]
ssdd - 24-11-2007 at 18:34
I didn't realize that those two compounds were so similar, pretty neat. This is why I find bio-chem so interesting because one compound can have a
host of effects in a multitude of things.
When I get the chance I'll have to read those links.....
-ssdd