CrossxD - 2-10-2016 at 12:04
Hello guys,
can you please help me?
I want ot make a lot of ammonia and cheapest is ammonia in urea ( I have about 25kg) and I need urease, I fount that urase is in soy beans or in peas,
is possible to extract it with common chemicals and without centrifuge?
CharlieA - 2-10-2016 at 12:20
If I remember correctly, where I worked ~50 years ago, urease was extracted from soy beans with water, filtered in a vacuum-filter press, and then
freeze-dried (by lyophilization).
Metacelsus - 2-10-2016 at 13:33
You might find this attached paper useful (in fact, it's the first-ever paper to describe crystallization of an enzyme).
You really should have a centrifuge, but it's not too hard to make one.
Attachment: J. Biol. Chem.-1926-Sumner-435-41.pdf (492kB)
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[Edited on 10-2-2016 by Metacelsus]