Romix - 8-5-2016 at 07:24
This lives in acidic conditions, after I fed it with sugar, it died.
Solution with it smells like nail vanish.
Grown in oxygen free environment.
Bakers yeast
Almost all died, do you know what to feed it with?
What about this ?
[Edited on 9-5-2016 by Romix]
Ozone - 8-5-2016 at 07:53
A scale would help, but otherwise, nice microscopy. What set up are you using?
Aside, the first looks like a co-fermentation of rods and cocci. The rods may be a Clostridium, perhaps acetylbutylicum (or the like) that are known
for acetone-butanol-ethanol (ABE) fermentation. Cocci are probably some facultative anaerobe, say Leuconostoc and friends.
Second and third look like haploid yeast and, maybe, spores (S. cerevisiae) at different magnification, and the 4th like diploid yeast (definitely
yeast). They may be the same organism, but in different stages of reproduction/environmental reaction: https://en.wikipedia.org/wiki/Mating_of_yeast
That they die with sucrose is odd, though--they usually don't have a problem with invertase (https://en.wikipedia.org/wiki/Invertase). It's hard to osmotically kill yeast (up to about 30% total sugar, anyway). But, you don't give any other
information on your media, so it's anyone's guess as to other nutrient requirements. Perhaps malt, maltose or saccharified starch would yield better
results? I'd check the nutrient requirements--they need more than sugar.
In any case, many years ago I observed a wild yeast growing on diluted cane molasses. The smell of acetone would blow your head off when you opened
the (5 gal) bucket. No attempt was made, at the time, to see if there was (undoubtedly) a co-fermentation, or with what (chip-based sequencing was
still a long ways out).
O3
Romix - 8-5-2016 at 08:10
Don't know condition they living in acidic, fourth or fifth circle with same water not changing. Interesting, adding more sugar every time and it
dissolving.
Not that much ol distills. Where does it go by mass.
[Edited on 8-5-2016 by Romix]
Romix - 8-5-2016 at 09:44
Uploading pictures of mold that grows on my walls under microscope.
20 - 30 min.
What cause it?
I saw mold ripping of citrate anions from salts.
Wall paint consists of oxides, there's no nutrients for mold, or I wrong?
Can dissolved in moist gases feed it?
Don't know how to get rid of it, tried everything.
[Edited on 8-5-2016 by Romix]
Romix - 8-5-2016 at 10:22
Ok, done.
https://youtu.be/UqMqH34Mx2M
Scrapped bit of mold of the wall, and left it in petri dish for month with a spoon of sugar added.
Check out creacher on 1.45.
[Edited on 9-5-2016 by Romix]
PHILOU Zrealone - 9-5-2016 at 04:47
Dust, spores and bacterias from air, insects excrements and finally minerals and moisture from wall (Na, K, Ca, SO4, NH4, NO3, H2O)
--> yes molds may grow on walls
Avoid this/get rid of it...
1°) Clean with detergent water, Detol solution, extended Javel water (bleach) and ethanol. And a sponge.
2°) Allow to dry (venting)
3°) Use white bitume paint (usually for outdoors but very effective for bathroom and indoor mold-ed walls), allow to dry and vent
Molds should not show for a long while...
of course:
-use a little venting (forced aspiration/venting, open Windows from time to time)
-allow some space between walls and furnitures and between furnitures... for air, heat (especially on cold walls favourizing condensation) and venting
to pass
-and do allow some heating of the room
[Edited on 9-5-2016 by PHILOU Zrealone]
Romix - 22-10-2022 at 12:26
Remember looking on mushroom spores under the microscope they used to look like American rugby balls of brown color.
Romix - 22-10-2022 at 12:28
Adding cigarettes ash, to the fermenting brew increases the yields of moonshine by much, also ferments much faster.
Just tablespoon of ash for every 5 liters of brew, does the trick.
Tsjerk - 22-10-2022 at 13:50
I didn't see this post before, what is the size of the cells?
Romix - 22-10-2022 at 13:58
No idea...
Romix - 22-10-2022 at 14:06
Had a microscope with which I could take pictures of the microbes and make videos.
Now same microscope cost over 300£, back then they used to sell for like 100 quid new on ebay.
When I came out from hospital, my right eye was shaking up and down. From over doing stimulants at work, not sleeping for weeks working and not eating
any food. And it wasn't an easy job me doing... Warehousing... Very hard.
Ok I thought it's the end of my chemistry carrier and sold all the glass I had, metal collection and microscope with it. Very cheap indeed. But after
a year of having SOM symptoms my eye stopped shaking and I returned to the hobby that I love.
[Edited on 22-10-2022 by Romix]