symboom - 31-3-2016 at 23:05
Ive been trying to look for info on converting cellulose to glucose ive read that cellulose is bonded glucose what could be used to separate the
chemical bonds and free the glucose sugar
Edit think I may have found this
Acid Hydrolysis (Sulfuric Acid): Use the same cellulose sources as in enzymatic hydrolysis.
Add 0.2g cellulose to 10ml of 5% H2SO4 solution in a lightly capped test tube. See Note 3. One may choose to carry out the reaction at 90°C instead
of at room temperature.
This reaction should last for 2 hours. Take 1 ml samples at some predetermined appropriate intervals.
Stop the hydrolysis reaction in the sample by neutralizing the acid and slightly reversing the pH with the addition of a small volume of a
concentrated potassium hydroxide solution. Make a quick calculation to see how much KOH is needed for this purpose. Note that one has to keep a close
track of the volume of KOH solution added because this information will be needed to calculate the glucose concentration in the original undiluted
sample.
Measure the glucose concentration of the alkaline sample.
Acid Hydrolysis (Hydrochloric Acid): Substitute 5% sulfuric acid with 5% hydrochloric acid and repeat the same procedures as in sulfuric acid.
Source
http://www.eng.umd.edu/~nsw/ench485/lab4.htm
[Edited on 1-4-2016 by symboom]