Hi,
Does anyone know or has anyone used diatomaceous earth for the solid phase in liquid chromatography?
Silicon gel one has jumped in price lately, I am trying to isolate samples from different plant extracts.
Cheersbyko3y - 25-9-2015 at 17:28
Buy a usual silica gel (beads), crush it with hammer, sift through a large sieve to remove really big particles. Obtained silica gel will have a
variable particle size, but is sufficient for simple chromatography.
[Edited on 26-9-2015 by byko3y]battoussai114 - 25-9-2015 at 18:04
It's used in GC from what I've heard. If you bother doing a little acid washing to ensure you only have Silica on your diatomaceous earth I'd guess
you'd be fine.Little_Ghost_again - 26-9-2015 at 01:48
It's used in GC from what I've heard. If you bother doing a little acid washing to ensure you only have Silica on your diatomaceous earth I'd guess
you'd be fine.
I will give that a go, silica seems to have gone to silly prices. I guess I could wash it and ball mill a bit. The stuff I was using is now £22 for
500g, seems steep when you get 5-6 columns out of it,
The cheaper stuff varies widely in particle size and hasnt given good separation. I did think of reusing the gell but everything I have seen and read
advises against this Little_Ghost_again - 26-9-2015 at 01:52
Buy a usual silica gel (beads), crush it with hammer, sift through a large sieve to remove really big particles. Obtained silica gel will have a
variable particle size, but is sufficient for simple chromatography.
[Edited on 26-9-2015 by byko3y]
this one http://www.ebay.co.uk/itm/8-KG-Silica-Gel-for-column-chromat...
is mesh 70-230 and £120 for 8Kg, so the commercial stuff varies widely in size anyway, if I could find the beads at a reasonable price I would ball
mill them, I dont get why its jumped so high in price over the last 4 months
