stain diferential of rough ER and smooth ER?

Quote: Originally posted by forgottenpassword

I know nothing about this whatsoever but I came up with "the lack of ribosomes means that the smooth ER is not basophilic and usually stains poorly by H&E." http://en.wikipedia.org/wiki/H%26E_stain http://medcell.med.yale.edu/histology/cell_lab/rough_endopla... [Edited on 28-11-2014 by forgottenpassword]

I need to stain the ER (both types) and in a perfect world would have a stain that only stains RER one colour and SER another. But its isnt a perfect world , the annoying thing is I have a solution using fluro stains and some phase contrast trickery. My problem is one I dont like the cost and two I dont like the faff!

But your statement that SER is not basophillic has given me an idea. I will try it out in the morning and see what happens . its at times like this I wish my bloody microscope camera attachment worked!!! I had a decent one for the Nikon camera and stupidly left it in the lab, now its dead. I might try and take it apart and fix it, or maybe buy a cheap attachment for the camera.
I have some superb yeast cell slides I would love to photograph and post. Also I do like to photograph my slides with a decent camera, you can bring it up on a huge monitor and really study the picture, I find it much better than just using the eye through the microscope. Modern High res camera's take microscopy onto another level. Shame the attachments cost so much. Thanks for the links they have got me thinking of another way around it.
LG