Sciencemadness Discussion Board

VSV terminator sequence

zinc finger - 6-4-2022 at 05:48

Hey there,

I am looking for the VSV terminator sequence and derivatives.
I was told, there should be lists of these sequences with corresponding properties like terminator strength.

Thanks in advance!

DrRadium - 1-8-2022 at 22:02

Quote: Originally posted by zinc finger  
Hey there,

I am looking for the VSV terminator sequence and derivatives.
I was told, there should be lists of these sequences with corresponding properties like terminator strength.

Thanks in advance!

The VSV consensus sequence is TATCTGTTAGTTTTTTT
A quick search revealed this interesting paper using different constructs using different arrangements of VSV ad PTH terminators for multigene expression in synthetic biology:
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3312007/
Full text is free

It might be a good place to start
You might also look at:https://pubmed.ncbi.nlm.nih.gov/12213662/
pdf is attached. It doesn't contain the table you want but you might be able to find what you need depending on exactly what you are looking for.
https://pubmed.ncbi.nlm.nih.gov/12213662/


Suggests that

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