Quote: Originally posted by VeritasC&E |
The HCl route sounds more interesting than resorting to the likes of HF.
@Unionised: When you write "for most amino acids" would you perhaps know to list amino acids this certainly excludes? Or list amino avids this
certainly includes? |
Well, I searched quickly, and that makes sense to me, that tryptophan will not stand these conditions, thats why a few percent of thioglycolic acid
are added to protect that.
Threonine and serine also can partially decompose during acidic hydrolysis.
Have a look here: https://www.researchgate.net/post/Can_anyone_recommend_a_sim...
Someone else there also recommend pepsin, and it is very cost effective apparently(20mg per gram of protein only), and its pretty mild and quick.
Basic hydrolysis is also possible, even in the microwave, and then it seems to be quite fast as well.
The link gives more thorough informations, I just summarised what I found there. |