Difference between revisions of "Haematoxylin"
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− | Haematoxylin | + | Haematoxylin also calledhematoxylin, 'natural black 1' or 'C.I. 75290' is a compound extracted from the heartwood of the logwood tree. Hematoxylin is a basic / positive compound that binds to and forms salts with acidic, or basophilic, compounds containing negative charges (such as DNA and RNA which are acidic/negative because the nucleic acid building blocks that come off the phosphate backbone are negatively charged) and stains them dark blue or violet. Haematoxylin and eosin together make up haematoxylin and eosin stain, one of the most commonly used stains in histology. This type of stain is a permanent stain as opposed to temporary stains (e.g. iodine solution in KI). Another common stain is phosphotungstic acid haematoxylin, a mix of haematoxylin with phosphotungstic acid. |
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Revision as of 13:14, 29 January 2016
Haematoxylin also calledhematoxylin, 'natural black 1' or 'C.I. 75290' is a compound extracted from the heartwood of the logwood tree. Hematoxylin is a basic / positive compound that binds to and forms salts with acidic, or basophilic, compounds containing negative charges (such as DNA and RNA which are acidic/negative because the nucleic acid building blocks that come off the phosphate backbone are negatively charged) and stains them dark blue or violet. Haematoxylin and eosin together make up haematoxylin and eosin stain, one of the most commonly used stains in histology. This type of stain is a permanent stain as opposed to temporary stains (e.g. iodine solution in KI). Another common stain is phosphotungstic acid haematoxylin, a mix of haematoxylin with phosphotungstic acid.
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IUPAC name
7,11b-Dihydroindeno[2,1-c]chromene-3,4,6a,9,10(6H)-pentol
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Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa). | |
Infobox references | |
(Introduction)
Contents
Properties
Chemical
Physical
Availability
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Preparation
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Projects
Handling
Safety
Storage
Disposal
References
https://en.m.wikipedia.org/wiki/Haematoxylin===Relevant Sciencemadness threads===