Measurements of the Ultraviolet Fluorescence Cross Sections and Spectra of Bacillus Anthracis Simulants.

Local PDF: ADA364911.pdf

AD Number: ADA364911
Subject Categories: MICROBIOLOGY OPTICS
Corporate Author: LOS ALAMOS NATIONAL LAB NM
Title: Measurements of the Ultraviolet Fluorescence Cross Sections and Spectra of Bacillus Anthracis Simulants.
Descriptive Note: Final rept. Jun 95-Sep 98
Personal Authors: Stephens, John R.
Report Date: MAY 1999
Pages: 48 PAGES
Report Number: LAUR-98-4173
Contract Number: W-7405-ENG-36
Monitor Acronym: ECBC, XA
Monitor Series: CR-004, ECBC
Descriptors: *ULTRAVIOLET RADIATION, *BACILLUS SUBTILIS, *BACILLUS ANTHRACIS, *BACILLUS CEREUS, PEAK VALUES, INTEGRATED SYSTEMS, FLUORESCENCE, SPECTROSCOPY, MATERIALS, ILLUMINATION, BACILLUS, SPORES, SPECTRA, PARTICLES, CROSS SECTIONS, FILTERS, VEGETATION, CELLS(BIOLOGY), POLLEN, DRYING, CHEMICAL AGENT SIMULANTS, ARC LAMPS.
Identifiers: AUTOFLUORESCENCE SPECTRA, BG(BACILLUS GLOBIGII), BM(BACILLUS MEGATERIUM), BC(BACILLUS CEREUS)
Abstract: Measurements of the ultraviolet autofluorescence spectra and absolute cross sections of the Bacillus anthracis (Ba) simulants Bacillus globigii (Bg), Bacillus megaterium (Bm), Bacillus subtilis (Bs), and Bacillus cereus (Bc) were measured. Fluorescence spectra and cross sections of pine pollen (Pins echinata) were measured for comparison. Both dried vegetative cells and spores separated from the sporulated vegetative material were studied. The spectra were obtained by suspending a small number (<10) of particles in air in our Single Particle Spectroscopy Apparatus (SPSA), illuminating the particles with light from a spectrally filtered arc lamp and measuring the fluorescence spectra of the particles. The illumination was 230 nm (20 nm FWHM) and the fluorescence spectra was measured between 300 and 450 nm. The fluorescence cross section of vegetative Bg peaks at 320 nm with a maximum cross section of 5 X 10-14 cm2/sr- nm- particle, whereas the Bg spore fluorescence peaks at 310 nm with peak fluorescence of 8 X 10-15 cm2/sr-nm-particle. Pine pollen particles showed a higher fluorescence peaking at 353 nm with a cross section of 1.7 X 10-13 cm2/ sr-nm-particle. Integrated cross sections ranged from 3.0 X 10-13 cm2/sr- particle for the Bg spores to 2.25 X 10-12 cm2/sr-particle for the vegetative cells.
Limitation Code: APPROVED FOR PUBLIC RELEASE
Source Code: 211350
Citation Creation Date: 07 JUL 1999